The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus

Ting Wei, Dongsheng Li, Daneth Marcial, Moshin Khan, Min-Hsuan Lin, Natale Snape, Reena GHILDYAL, David Harrich, Kirsten Spann

Research output: Contribution to journalArticle

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Abstract

The eukaryotic translation factor eEF1A assists replication of many RNA viruses by various mechanisms. Here we show that down-regulation of eEF1A restricts the expression of viral genomic RNA and the release of infectious virus, demonstrating a biological requirement for eEF1A in the respiratory syncytial virus (RSV) life cycle. The key proteins in the replicase/transcriptase complex of RSV; the nucleocapsid (N) protein, phosphoprotein (P) and matrix (M) protein, all associate with eEF1A in RSV infected cells, although N is the strongest binding partner. Using individually expressed proteins, N, but not P or M bound to eEF1A. This study demonstrates a novel interaction between eEF1A and the RSV replication complex, through binding to N protein, to facilitate genomic RNA synthesis and virus production.
Original languageEnglish
Article numbere114447
Pages (from-to)1-14
Number of pages14
JournalPLoS One
Volume9
Issue number12
DOIs
Publication statusPublished - 2014

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Respirovirus
Peptide Elongation Factors
Respiratory Syncytial Viruses
Viruses
Genes
Genome
viruses
genome
RNA Viruses
Proteins
proteins
Virus Release
Nucleocapsid Proteins
RNA
Phosphoproteins
Viral RNA
genomics
DNA-Directed RNA Polymerases
Virus Replication
Life Cycle Stages

Cite this

Wei, Ting ; Li, Dongsheng ; Marcial, Daneth ; Khan, Moshin ; Lin, Min-Hsuan ; Snape, Natale ; GHILDYAL, Reena ; Harrich, David ; Spann, Kirsten. / The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus. In: PLoS One. 2014 ; Vol. 9, No. 12. pp. 1-14.
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Wei, T, Li, D, Marcial, D, Khan, M, Lin, M-H, Snape, N, GHILDYAL, R, Harrich, D & Spann, K 2014, 'The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus', PLoS One, vol. 9, no. 12, e114447, pp. 1-14. https://doi.org/10.1371/journal.pone.0114447

The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus. / Wei, Ting; Li, Dongsheng; Marcial, Daneth; Khan, Moshin; Lin, Min-Hsuan; Snape, Natale; GHILDYAL, Reena; Harrich, David; Spann, Kirsten.

In: PLoS One, Vol. 9, No. 12, e114447, 2014, p. 1-14.

Research output: Contribution to journalArticle

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AU - Wei, Ting

AU - Li, Dongsheng

AU - Marcial, Daneth

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AU - Lin, Min-Hsuan

AU - Snape, Natale

AU - GHILDYAL, Reena

AU - Harrich, David

AU - Spann, Kirsten

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