The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus

Ting Wei, Dongsheng Li, Daneth Marcial, Moshin Khan, Min-Hsuan Lin, Natale Snape, Reena GHILDYAL, David Harrich, Kirsten Spann

    Research output: Contribution to journalArticle

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    Abstract

    The eukaryotic translation factor eEF1A assists replication of many RNA viruses by various mechanisms. Here we show that down-regulation of eEF1A restricts the expression of viral genomic RNA and the release of infectious virus, demonstrating a biological requirement for eEF1A in the respiratory syncytial virus (RSV) life cycle. The key proteins in the replicase/transcriptase complex of RSV; the nucleocapsid (N) protein, phosphoprotein (P) and matrix (M) protein, all associate with eEF1A in RSV infected cells, although N is the strongest binding partner. Using individually expressed proteins, N, but not P or M bound to eEF1A. This study demonstrates a novel interaction between eEF1A and the RSV replication complex, through binding to N protein, to facilitate genomic RNA synthesis and virus production.
    Original languageEnglish
    Pages (from-to)1-14
    Number of pages14
    JournalPLoS One
    Volume9
    Issue number12
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Respirovirus
    Peptide Elongation Factors
    Respiratory Syncytial Viruses
    Viruses
    Genes
    Genome
    viruses
    genome
    RNA Viruses
    Proteins
    proteins
    Virus Release
    Nucleocapsid Proteins
    RNA
    Phosphoproteins
    Viral RNA
    genomics
    DNA-Directed RNA Polymerases
    Virus Replication
    Life Cycle Stages

    Cite this

    Wei, Ting ; Li, Dongsheng ; Marcial, Daneth ; Khan, Moshin ; Lin, Min-Hsuan ; Snape, Natale ; GHILDYAL, Reena ; Harrich, David ; Spann, Kirsten. / The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus. In: PLoS One. 2014 ; Vol. 9, No. 12. pp. 1-14.
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    The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus. / Wei, Ting; Li, Dongsheng; Marcial, Daneth; Khan, Moshin; Lin, Min-Hsuan; Snape, Natale; GHILDYAL, Reena; Harrich, David; Spann, Kirsten.

    In: PLoS One, Vol. 9, No. 12, 2014, p. 1-14.

    Research output: Contribution to journalArticle

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    AU - Lin, Min-Hsuan

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