The influence of bacteria on the arsenic species produced by laboratory cultures of the marine phytoplankton Dunaliella tertiolecta

Bill MAHER, Simon FOSTER, Katarina Mikac

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    Abstract

    To assess whether bacteria influence the biogeochemical cycling of arsenic by laboratory cultures of the marine phytoplankton Dunaliella tertiolecta, the arsenic species produced by D. tertiolecta were compared in “operationally sterile” and bacteria spiked cultures. It was observed that glycerol (Gly-) arsenoriboside (41–78 %), phosphate (PO4-) arsenoriboside (7–38 %) and arsenate (As(V)) (15–21 %) were the major water-soluble arsenic species in all D. tertiolecta cultures irrespective of whether cultures were operationally sterile or contained added bacteria. PO4-riboside (46–74 %) and Gly-riboside (24–36 %) were also the major arsenic species in hydrolysed lipid extracts of D. tertiolecta irrespective of whether cultures were operationally sterile or contained bacteria. In addition to similarities in the arsenic species produced, total arsenic concentrations and culture growth did not differ relative to whether cultures were operationally sterile or not. Similar bacterial strains were identified in all D. tertiolecta cultures irrespective of whether bacteria were added or not. Consequently, it is evident that the presence of “foreign” or “added” bacteria in D. tertiolecta has minimal influence on the metabolism and cycling of arsenic by phytoplankton. Thus, the use of laboratory phytoplankton cultures containing bacteria may be appropriate means to investigate arsenic biogeochemical cycling unlike previously believed.
    Original languageEnglish
    Pages (from-to)2129-2134
    Number of pages6
    JournalJournal of Applied Phycology
    Volume26
    Issue number5
    DOIs
    Publication statusPublished - 2014

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    Dunaliella tertiolecta
    arsenic
    phytoplankton
    bacterium
    bacteria
    glycerol
    arsenates
    laboratory
    arsenate
    metabolism
    lipid
    phosphate
    phosphates
    extracts

    Cite this

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    title = "The influence of bacteria on the arsenic species produced by laboratory cultures of the marine phytoplankton Dunaliella tertiolecta",
    abstract = "To assess whether bacteria influence the biogeochemical cycling of arsenic by laboratory cultures of the marine phytoplankton Dunaliella tertiolecta, the arsenic species produced by D. tertiolecta were compared in “operationally sterile” and bacteria spiked cultures. It was observed that glycerol (Gly-) arsenoriboside (41–78 {\%}), phosphate (PO4-) arsenoriboside (7–38 {\%}) and arsenate (As(V)) (15–21 {\%}) were the major water-soluble arsenic species in all D. tertiolecta cultures irrespective of whether cultures were operationally sterile or contained added bacteria. PO4-riboside (46–74 {\%}) and Gly-riboside (24–36 {\%}) were also the major arsenic species in hydrolysed lipid extracts of D. tertiolecta irrespective of whether cultures were operationally sterile or contained bacteria. In addition to similarities in the arsenic species produced, total arsenic concentrations and culture growth did not differ relative to whether cultures were operationally sterile or not. Similar bacterial strains were identified in all D. tertiolecta cultures irrespective of whether bacteria were added or not. Consequently, it is evident that the presence of “foreign” or “added” bacteria in D. tertiolecta has minimal influence on the metabolism and cycling of arsenic by phytoplankton. Thus, the use of laboratory phytoplankton cultures containing bacteria may be appropriate means to investigate arsenic biogeochemical cycling unlike previously believed.",
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    author = "Bill MAHER and Simon FOSTER and Katarina Mikac",
    year = "2014",
    doi = "10.1007/s10811-014-0255-0",
    language = "English",
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    pages = "2129--2134",
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    TY - JOUR

    T1 - The influence of bacteria on the arsenic species produced by laboratory cultures of the marine phytoplankton Dunaliella tertiolecta

    AU - MAHER, Bill

    AU - FOSTER, Simon

    AU - Mikac, Katarina

    PY - 2014

    Y1 - 2014

    N2 - To assess whether bacteria influence the biogeochemical cycling of arsenic by laboratory cultures of the marine phytoplankton Dunaliella tertiolecta, the arsenic species produced by D. tertiolecta were compared in “operationally sterile” and bacteria spiked cultures. It was observed that glycerol (Gly-) arsenoriboside (41–78 %), phosphate (PO4-) arsenoriboside (7–38 %) and arsenate (As(V)) (15–21 %) were the major water-soluble arsenic species in all D. tertiolecta cultures irrespective of whether cultures were operationally sterile or contained added bacteria. PO4-riboside (46–74 %) and Gly-riboside (24–36 %) were also the major arsenic species in hydrolysed lipid extracts of D. tertiolecta irrespective of whether cultures were operationally sterile or contained bacteria. In addition to similarities in the arsenic species produced, total arsenic concentrations and culture growth did not differ relative to whether cultures were operationally sterile or not. Similar bacterial strains were identified in all D. tertiolecta cultures irrespective of whether bacteria were added or not. Consequently, it is evident that the presence of “foreign” or “added” bacteria in D. tertiolecta has minimal influence on the metabolism and cycling of arsenic by phytoplankton. Thus, the use of laboratory phytoplankton cultures containing bacteria may be appropriate means to investigate arsenic biogeochemical cycling unlike previously believed.

    AB - To assess whether bacteria influence the biogeochemical cycling of arsenic by laboratory cultures of the marine phytoplankton Dunaliella tertiolecta, the arsenic species produced by D. tertiolecta were compared in “operationally sterile” and bacteria spiked cultures. It was observed that glycerol (Gly-) arsenoriboside (41–78 %), phosphate (PO4-) arsenoriboside (7–38 %) and arsenate (As(V)) (15–21 %) were the major water-soluble arsenic species in all D. tertiolecta cultures irrespective of whether cultures were operationally sterile or contained added bacteria. PO4-riboside (46–74 %) and Gly-riboside (24–36 %) were also the major arsenic species in hydrolysed lipid extracts of D. tertiolecta irrespective of whether cultures were operationally sterile or contained bacteria. In addition to similarities in the arsenic species produced, total arsenic concentrations and culture growth did not differ relative to whether cultures were operationally sterile or not. Similar bacterial strains were identified in all D. tertiolecta cultures irrespective of whether bacteria were added or not. Consequently, it is evident that the presence of “foreign” or “added” bacteria in D. tertiolecta has minimal influence on the metabolism and cycling of arsenic by phytoplankton. Thus, the use of laboratory phytoplankton cultures containing bacteria may be appropriate means to investigate arsenic biogeochemical cycling unlike previously believed.

    KW - Arsenic species

    KW - Dunaliella tertiolecta

    KW - Bacteria

    KW - Laboratory cultures

    KW - Arsenoribosides.

    U2 - 10.1007/s10811-014-0255-0

    DO - 10.1007/s10811-014-0255-0

    M3 - Article

    VL - 26

    SP - 2129

    EP - 2134

    JO - Journal of Applied Phycology

    JF - Journal of Applied Phycology

    SN - 0921-8971

    IS - 5

    ER -