The lipid peroxidation product 4-hydroxynonenal contributes to oxidative stress-mediated deterioration of the ageing oocyte

Bettina P. Mihalas, Geoffry N. De Iuliis, Kate A. Redgrove, Eileen A. McLaughlin, Brett Nixon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

An increase in intraovarian reactive oxygen species (ROS) has long been implicated in the decline in oocyte quality associated with maternal ageing. Oxidative stress (OS)-induced lipid peroxidation and the consequent generation of highly electrophilic aldehydes, such as 4-hydroxynonenal (4-HNE), represents a potential mechanism by which ROS can inflict damage in the ageing oocyte. In this study, we have established that aged oocytes are vulnerable to damage by 4-HNE resulting from increased cytosolic ROS production within the oocyte itself. Further, we demonstrated that the age-related induction of OS can be recapitulated by exposure of germinal vesicle (GV) oocytes to exogenous H2 O2 . Such treatments stimulated an increase in 4-HNE generation, which remained elevated during in vitro oocyte maturation to metaphase II. Additionally, exposure of GV oocytes to either H2 O2 or 4-HNE resulted in decreased meiotic completion, increased spindle abnormalities, chromosome misalignments and aneuploidy. In seeking to account for these data, we revealed that proteins essential for oocyte health and meiotic development, namely α-, β-, and γ-tubulin are vulnerable to adduction via 4-HNE. Importantly, 4-HNE-tubulin adduction, as well as increased aneuploidy rates, were resolved by co-treatment with the antioxidant penicillamine, demonstrating a possible therapeutic mechanism to improve oocyte quality in older females.

Original languageEnglish
Article number6247
Pages (from-to)1-18
Number of pages18
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 24 Jul 2017
Externally publishedYes

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Lipid Peroxidation
Oocytes
Oxidative Stress
Reactive Oxygen Species
Aneuploidy
Tubulin
In Vitro Oocyte Maturation Techniques
Penicillamine
4-hydroxy-2-nonenal
Metaphase
Aldehydes
Chromosome Aberrations
Therapeutics
Antioxidants
Mothers
Health
Proteins

Cite this

Mihalas, Bettina P. ; De Iuliis, Geoffry N. ; Redgrove, Kate A. ; McLaughlin, Eileen A. ; Nixon, Brett. / The lipid peroxidation product 4-hydroxynonenal contributes to oxidative stress-mediated deterioration of the ageing oocyte. In: Scientific Reports. 2017 ; Vol. 7, No. 1. pp. 1-18.
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The lipid peroxidation product 4-hydroxynonenal contributes to oxidative stress-mediated deterioration of the ageing oocyte. / Mihalas, Bettina P.; De Iuliis, Geoffry N.; Redgrove, Kate A.; McLaughlin, Eileen A.; Nixon, Brett.

In: Scientific Reports, Vol. 7, No. 1, 6247, 24.07.2017, p. 1-18.

Research output: Contribution to journalArticle

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