The matrix protein of Human respiratory syncytial virus localises to the nucleus of infected cells and inhibits transcription

R Ghildyal, C Baulch-Brown, J Mills, J Meanger

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We studied the kinetics of localisation of matrix (M) protein of Human respiratory syncytial virus (RSV) in infected cells. M protein was detected in the nucleus early in infection, by confocal microscopy and by immunoblotting of nuclear fractions. We next tested the possibility that M protein may be involved in inhibition of host cell transcription. Nuclear extracts from RSV infected cells had less transcriptional activity in vitro when compared to nuclear extracts from mock infected cells. In addition, nuclear extracts from RSV infected cells inhibited the transcriptional activity of nuclear extracts from mock infected cells, suggesting that an inhibitory activity was transferred with nuclear extracts from RSV infected cells. Our data suggest that M protein may play a role early in the infection by inhibiting host cell transcription.

Original languageEnglish
Pages (from-to)1419-29
Number of pages11
JournalArchiv fur die gesamte Virusforschung
Volume148
Issue number7
DOIs
Publication statusPublished - Jul 2003
Externally publishedYes

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Human respiratory syncytial virus
Cell Nucleus
Respiratory Syncytial Viruses
Proteins
Infection
Immunoblotting
Confocal Microscopy

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title = "The matrix protein of Human respiratory syncytial virus localises to the nucleus of infected cells and inhibits transcription",
abstract = "We studied the kinetics of localisation of matrix (M) protein of Human respiratory syncytial virus (RSV) in infected cells. M protein was detected in the nucleus early in infection, by confocal microscopy and by immunoblotting of nuclear fractions. We next tested the possibility that M protein may be involved in inhibition of host cell transcription. Nuclear extracts from RSV infected cells had less transcriptional activity in vitro when compared to nuclear extracts from mock infected cells. In addition, nuclear extracts from RSV infected cells inhibited the transcriptional activity of nuclear extracts from mock infected cells, suggesting that an inhibitory activity was transferred with nuclear extracts from RSV infected cells. Our data suggest that M protein may play a role early in the infection by inhibiting host cell transcription.",
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The matrix protein of Human respiratory syncytial virus localises to the nucleus of infected cells and inhibits transcription. / Ghildyal, R; Baulch-Brown, C; Mills, J; Meanger, J.

In: Archiv fur die gesamte Virusforschung, Vol. 148, No. 7, 07.2003, p. 1419-29.

Research output: Contribution to journalArticle

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T1 - The matrix protein of Human respiratory syncytial virus localises to the nucleus of infected cells and inhibits transcription

AU - Ghildyal, R

AU - Baulch-Brown, C

AU - Mills, J

AU - Meanger, J

PY - 2003/7

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KW - Binding Sites

KW - Carcinoma, Hepatocellular

KW - Cell Nucleus

KW - Gene Expression Regulation, Viral

KW - Humans

KW - Kinetics

KW - Liver Neoplasms

KW - Respiratory Syncytial Virus, Human

KW - Transcription, Genetic

KW - Tumor Cells, Cultured

KW - Viral Matrix Proteins

KW - Viral Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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JO - Archiv fur die gesamte Virusforschung

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