The Role Of Osmolality In Amphibian Sperm Cryopreservation; A Radical New Paradigm

Rose Upton; Simon Clulow; Michael Mahony; John Clulow

doi:10.1016/j.cryobiol.2019.10.124

The Role Of Osmolality In Amphibian Sperm Cryopreservation; A Radical New Paradigm

Rose Upton, Simon Clulow, Michael Mahony, John Clulow

Research output: Contribution to journal › Meeting Abstract › peer-review

Abstract

Amphibians are experiencing global declines, rendering artificial reproductive technologies of great interest. Little attention is given in the literature to the non-penetrating component of amphibian sperm cryodiluents; these are typically iso- or hypertonic. In this study the non-penetrating component of the cryodiluent (sucrose) was tested at two concentrations; 1 and 10% w/v, in combination with either 10 or 15% v/v dimethyl sulfoxide (Me2SO) in Litoria dentata (n=4). The responses of motility (non-motile, non-progressive and forward-progressive motility) and vitality were assessed.

Original language	English
Pages (from-to)	177-177
Number of pages	1
Journal	Cryobiology
Volume	91
DOIs	https://doi.org/10.1016/j.cryobiol.2019.10.124
Publication status	Published - Dec 2019
Externally published	Yes
Event	CRYO2019: 56th Annual Meeting of the Society for Cryobiology - San Diego, San Diego, United States Duration: 22 Jul 2019 → 25 Jul 2019

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10.1016/j.cryobiol.2019.10.124

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title = "The Role Of Osmolality In Amphibian Sperm Cryopreservation; A Radical New Paradigm",

abstract = "Amphibians are experiencing global declines, rendering artificial reproductive technologies of great interest. Little attention is given in the literature to the non-penetrating component of amphibian sperm cryodiluents; these are typically iso- or hypertonic. In this study the non-penetrating component of the cryodiluent (sucrose) was tested at two concentrations; 1 and 10% w/v, in combination with either 10 or 15% v/v dimethyl sulfoxide (Me2SO) in Litoria dentata (n=4). The responses of motility (non-motile, non-progressive and forward-progressive motility) and vitality were assessed. ",

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note = "CRYO2019: 56th Annual Meeting of the Society for Cryobiology, CRYO2019 ; Conference date: 22-07-2019 Through 25-07-2019",

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AU - Clulow, Simon

AU - Mahony, Michael

AU - Clulow, John

PY - 2019/12

Y1 - 2019/12

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AB - Amphibians are experiencing global declines, rendering artificial reproductive technologies of great interest. Little attention is given in the literature to the non-penetrating component of amphibian sperm cryodiluents; these are typically iso- or hypertonic. In this study the non-penetrating component of the cryodiluent (sucrose) was tested at two concentrations; 1 and 10% w/v, in combination with either 10 or 15% v/v dimethyl sulfoxide (Me2SO) in Litoria dentata (n=4). The responses of motility (non-motile, non-progressive and forward-progressive motility) and vitality were assessed.

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JO - Cryobiology

JF - Cryobiology

T2 - CRYO2019: 56th Annual Meeting of the Society for Cryobiology

Y2 - 22 July 2019 through 25 July 2019

ER -

The Role Of Osmolality In Amphibian Sperm Cryopreservation; A Radical New Paradigm

Abstract

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