The role of protein kinase-C theta in control of epithelial to mesenchymal transition and cancer stem cell formation

Anjum ZAFAR, Kris HARDY, Jasmine Li, Fan WU, Sudha RAO

Research output: Contribution to journalArticle

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Abstract

The protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) induces transition of the epithelial MCF-7 cell line to a mesenchymal phenotype. A subset of the resulting mesenchymal cells has surface markers characteristics of a cancer stem cell (CSC) population. We profiled the transcriptome changes associated with the epithelial to mesenchymal transition and those that occurred in the CSC subset. Using a siRNA knockdown strategy, we examined the extent to which these changes were dependent on the PKC family member, PKC-¿. The importance of the cytoplasmic signaling role of this kinase is well established and in this study, we have shown by PKC-¿ ChIP-sequencing analysis that this kinase has a dual role with the ability to also associate with chromatin on a subset of PKC-¿ dependent genes. In the associated manuscript (Zafar et al., 2014 [5]) we presented evidence for the first time showing that this nuclear role of PKC-¿ is also important for gene induction and mesenchymal/CSC phenotype. Here we describe the analysis associated with the transcriptome and ChIP-seq data presented in Zafar et al. (2014) [5] and uploaded to NCBI Gene Expression Omnibus (. GSE53335).
Original languageEnglish
Pages (from-to)28-32
Number of pages5
JournalGenomics Data
Volume3
Issue number1
DOIs
Publication statusPublished - Mar 2015

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Epithelial-Mesenchymal Transition
Neoplastic Stem Cells
Stem cells
Mesenchymal Stromal Cells
Protein Kinase C
Transcriptome
Phosphotransferases
Genes
Phenotype
Aptitude
Manuscripts
MCF-7 Cells
Nuclear Proteins
Gene expression
Small Interfering RNA
Chromatin
Acetates
Epithelial Cells
Cells
Gene Expression

Cite this

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abstract = "The protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) induces transition of the epithelial MCF-7 cell line to a mesenchymal phenotype. A subset of the resulting mesenchymal cells has surface markers characteristics of a cancer stem cell (CSC) population. We profiled the transcriptome changes associated with the epithelial to mesenchymal transition and those that occurred in the CSC subset. Using a siRNA knockdown strategy, we examined the extent to which these changes were dependent on the PKC family member, PKC-¿. The importance of the cytoplasmic signaling role of this kinase is well established and in this study, we have shown by PKC-¿ ChIP-sequencing analysis that this kinase has a dual role with the ability to also associate with chromatin on a subset of PKC-¿ dependent genes. In the associated manuscript (Zafar et al., 2014 [5]) we presented evidence for the first time showing that this nuclear role of PKC-¿ is also important for gene induction and mesenchymal/CSC phenotype. Here we describe the analysis associated with the transcriptome and ChIP-seq data presented in Zafar et al. (2014) [5] and uploaded to NCBI Gene Expression Omnibus (. GSE53335).",
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The role of protein kinase-C theta in control of epithelial to mesenchymal transition and cancer stem cell formation. / ZAFAR, Anjum; HARDY, Kris; Li, Jasmine; WU, Fan; RAO, Sudha.

In: Genomics Data, Vol. 3, No. 1, 03.2015, p. 28-32.

Research output: Contribution to journalArticle

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