TY - JOUR
T1 - The snakeskin gourami (Trichopodus pectoralis) tends to exhibit xx/xy sex determination
AU - Panthum, Thitipong
AU - Laopichienpong, Nararat
AU - Kraichak, Ekaphan
AU - Singchat, Worapong
AU - Nguyen, Dung Ho My
AU - Ariyaraphong, Nattakan
AU - Ahmad, Syed Farhan
AU - Muangmai, Narongrit
AU - Duengkae, Prateep
AU - Peyachoknagul, Surin
AU - Ezaz, Tariq
AU - Srikulnath, Kornsorn
N1 - Funding Information:
Funding: This research was financially supported in part by the Graduate Scholarship Program of the Graduate School, Kasetsart University, Thailand awarded to T.P. and K.S., the Office of the Ministry of Higher Education, Science, Research and Innovation; and the Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program 2021 awarded to T.P. and K.S., the Royal Golden Jubilee Ph.D. program under the Thailand Research Fund (TRF) awarded to D.H.M.N. and K.S. (No. PHD01392561), the Thailand Research Fund (TRF) (No. RSA6180075) provided through a grant awarded to K.S., a Postdoctoral Researcher award at Kasetsart University awarded to S.F.A. and K.S., and International SciKU Branding (ISB), Faculty of Science, Kasetsart University awarded funds to K.S. No funding source was involved in the study design, collection, analysis and interpretation of the data, writing the report and the decision to submit the article for publication.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - The snakeskin gourami (Trichopodus pectoralis) has a high meat yield and is one of the top five aquaculture freshwater fishes in Thailand. The species is not externally sexually dimorphic, and its sex determination system is unknown. Understanding the sex determination system of this species will contribute to its full-scale commercialization. In this study, a cytogenetic analysis did not reveal any between-sex differences in chromosomal patterns. However, we used genotyping-by-sequencing to identify 4 male-linked loci and 1 female-linked locus, indicating that the snakeskin gourami tends to exhibit an XX/XY sex determination system. However, we did not find any male-specific loci after filtering the loci for a ratio of 100:0 ratio of males:females. This suggests that the putative Y chromosome is young and that the sex determination region is cryptic. This approach provides solid information that can help identify the sex determination mechanism and potential sex determination regions in the snakeskin gourami, allowing further investigation of genetic improvements in the species.
AB - The snakeskin gourami (Trichopodus pectoralis) has a high meat yield and is one of the top five aquaculture freshwater fishes in Thailand. The species is not externally sexually dimorphic, and its sex determination system is unknown. Understanding the sex determination system of this species will contribute to its full-scale commercialization. In this study, a cytogenetic analysis did not reveal any between-sex differences in chromosomal patterns. However, we used genotyping-by-sequencing to identify 4 male-linked loci and 1 female-linked locus, indicating that the snakeskin gourami tends to exhibit an XX/XY sex determination system. However, we did not find any male-specific loci after filtering the loci for a ratio of 100:0 ratio of males:females. This suggests that the putative Y chromosome is young and that the sex determination region is cryptic. This approach provides solid information that can help identify the sex determination mechanism and potential sex determination regions in the snakeskin gourami, allowing further investigation of genetic improvements in the species.
KW - Cryptic sex chromosome
KW - Fluorescence in situ hybridization
KW - Polygenic sex determination
KW - Recombination
KW - Sex determination
KW - Single nucleotide polymorphism
KW - Teleost
KW - Trichopodus pectoralis
UR - http://www.scopus.com/inward/record.url?scp=85116100464&partnerID=8YFLogxK
U2 - 10.3390/fishes6040043
DO - 10.3390/fishes6040043
M3 - Article
AN - SCOPUS:85116100464
VL - 6
SP - 1
EP - 17
JO - Fishes
JF - Fishes
IS - 4
M1 - 43
ER -