The use of zirconium stationary phase to determine the four major arsenoriboses concentrations in marine organisms by high-performance liquid chromatography coupled to inductively coupled plasma–mass spectrometry

Eman Alkasasbeh, William A. Maher, Frady G. Adly, Simon Foster, Frank Krikowa

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Abstract

HPLC-ICP-MS utilising a ZirChrom®-SAX column with an aqueous 10 mM ammonium dihydrogen phosphate mobile phase at pH 7.5 (adjusted with aqueous ammonia) was evaluated for measuring glycerol arsenoriboside (OH-ribose), phosphate arsenoriboside (PO4-ribose), sulfonate arsenoriboside (SO3-ribose) and sulfate arsenoriboside (OSO3-ribose) in marine macroalgae and animals by analysis of seven reference materials. The results obtained were compared to those obtained using PRP-X100 anion-exchange chromatography. Inorganic arsenic species were not eluted from the zirconium column due to the strong interaction with the zirconia stationary phase. Measurements of SO3-ribose and OSO3-ribose concentrations were in close agreement with those obtained using the PRP-X100 column with a 0.005 mg As kg−1 detection limit. Peak shapes of these arsenoriboses were improved allowing lower amounts to be quantified. The quantification of OH-ribose and PO4-ribose, however, was sample dependent and not possible if arsenobetaine (AB) and (DMA) were present at large concentrations in samples because of coelution of these species with OH-ribose and PO4-ribose, respectively. In the absence of OH-ribose, AB can be determined using the zirconium column while AB on the PRP-X100 column coelutes with arsenic cations.

Original languageEnglish
Article number104099
Pages (from-to)1-11
Number of pages11
JournalMicrochemical Journal
Volume150
DOIs
Publication statusPublished - Nov 2019

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Ribose
High performance liquid chromatography
Spectrometry
Arsenic
Sulfates
Phosphates
Dynamic mechanical analysis
Chromatography
Ammonium Compounds
Ammonia
Glycerol
Anions
Cations
Animals

Cite this

@article{2891ceceec09422dadc1ec52f37587b2,
title = "The use of zirconium stationary phase to determine the four major arsenoriboses concentrations in marine organisms by high-performance liquid chromatography coupled to inductively coupled plasma–mass spectrometry",
abstract = "HPLC-ICP-MS utilising a ZirChrom{\circledR}-SAX column with an aqueous 10 mM ammonium dihydrogen phosphate mobile phase at pH 7.5 (adjusted with aqueous ammonia) was evaluated for measuring glycerol arsenoriboside (OH-ribose), phosphate arsenoriboside (PO4-ribose), sulfonate arsenoriboside (SO3-ribose) and sulfate arsenoriboside (OSO3-ribose) in marine macroalgae and animals by analysis of seven reference materials. The results obtained were compared to those obtained using PRP-X100 anion-exchange chromatography. Inorganic arsenic species were not eluted from the zirconium column due to the strong interaction with the zirconia stationary phase. Measurements of SO3-ribose and OSO3-ribose concentrations were in close agreement with those obtained using the PRP-X100 column with a 0.005 mg As kg−1 detection limit. Peak shapes of these arsenoriboses were improved allowing lower amounts to be quantified. The quantification of OH-ribose and PO4-ribose, however, was sample dependent and not possible if arsenobetaine (AB) and (DMA) were present at large concentrations in samples because of coelution of these species with OH-ribose and PO4-ribose, respectively. In the absence of OH-ribose, AB can be determined using the zirconium column while AB on the PRP-X100 column coelutes with arsenic cations.",
keywords = "Arsenoriboses, HPLC-ICP-MS, Marine algae, Marine animals, Speciation, Zirconia",
author = "Eman Alkasasbeh and Maher, {William A.} and Adly, {Frady G.} and Simon Foster and Frank Krikowa",
year = "2019",
month = "11",
doi = "10.1016/j.microc.2019.104099",
language = "English",
volume = "150",
pages = "1--11",
journal = "Microchemical Journal",
issn = "0026-265X",
publisher = "Elsevier Inc.",

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TY - JOUR

T1 - The use of zirconium stationary phase to determine the four major arsenoriboses concentrations in marine organisms by high-performance liquid chromatography coupled to inductively coupled plasma–mass spectrometry

AU - Alkasasbeh, Eman

AU - Maher, William A.

AU - Adly, Frady G.

AU - Foster, Simon

AU - Krikowa, Frank

PY - 2019/11

Y1 - 2019/11

N2 - HPLC-ICP-MS utilising a ZirChrom®-SAX column with an aqueous 10 mM ammonium dihydrogen phosphate mobile phase at pH 7.5 (adjusted with aqueous ammonia) was evaluated for measuring glycerol arsenoriboside (OH-ribose), phosphate arsenoriboside (PO4-ribose), sulfonate arsenoriboside (SO3-ribose) and sulfate arsenoriboside (OSO3-ribose) in marine macroalgae and animals by analysis of seven reference materials. The results obtained were compared to those obtained using PRP-X100 anion-exchange chromatography. Inorganic arsenic species were not eluted from the zirconium column due to the strong interaction with the zirconia stationary phase. Measurements of SO3-ribose and OSO3-ribose concentrations were in close agreement with those obtained using the PRP-X100 column with a 0.005 mg As kg−1 detection limit. Peak shapes of these arsenoriboses were improved allowing lower amounts to be quantified. The quantification of OH-ribose and PO4-ribose, however, was sample dependent and not possible if arsenobetaine (AB) and (DMA) were present at large concentrations in samples because of coelution of these species with OH-ribose and PO4-ribose, respectively. In the absence of OH-ribose, AB can be determined using the zirconium column while AB on the PRP-X100 column coelutes with arsenic cations.

AB - HPLC-ICP-MS utilising a ZirChrom®-SAX column with an aqueous 10 mM ammonium dihydrogen phosphate mobile phase at pH 7.5 (adjusted with aqueous ammonia) was evaluated for measuring glycerol arsenoriboside (OH-ribose), phosphate arsenoriboside (PO4-ribose), sulfonate arsenoriboside (SO3-ribose) and sulfate arsenoriboside (OSO3-ribose) in marine macroalgae and animals by analysis of seven reference materials. The results obtained were compared to those obtained using PRP-X100 anion-exchange chromatography. Inorganic arsenic species were not eluted from the zirconium column due to the strong interaction with the zirconia stationary phase. Measurements of SO3-ribose and OSO3-ribose concentrations were in close agreement with those obtained using the PRP-X100 column with a 0.005 mg As kg−1 detection limit. Peak shapes of these arsenoriboses were improved allowing lower amounts to be quantified. The quantification of OH-ribose and PO4-ribose, however, was sample dependent and not possible if arsenobetaine (AB) and (DMA) were present at large concentrations in samples because of coelution of these species with OH-ribose and PO4-ribose, respectively. In the absence of OH-ribose, AB can be determined using the zirconium column while AB on the PRP-X100 column coelutes with arsenic cations.

KW - Arsenoriboses

KW - HPLC-ICP-MS

KW - Marine algae

KW - Marine animals

KW - Speciation

KW - Zirconia

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U2 - 10.1016/j.microc.2019.104099

DO - 10.1016/j.microc.2019.104099

M3 - Article

VL - 150

SP - 1

EP - 11

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

M1 - 104099

ER -