Abstract
HPLC-ICP-MS utilising a ZirChrom®-SAX column with an aqueous 10 mM ammonium dihydrogen phosphate mobile phase at pH 7.5 (adjusted with aqueous ammonia) was evaluated for measuring glycerol arsenoriboside (OH-ribose), phosphate arsenoriboside (PO4-ribose), sulfonate arsenoriboside (SO3-ribose) and sulfate arsenoriboside (OSO3-ribose) in marine macroalgae and animals by analysis of seven reference materials. The results obtained were compared to those obtained using PRP-X100 anion-exchange chromatography. Inorganic arsenic species were not eluted from the zirconium column due to the strong interaction with the zirconia stationary phase. Measurements of SO3-ribose and OSO3-ribose concentrations were in close agreement with those obtained using the PRP-X100 column with a 0.005 mg As kg−1 detection limit. Peak shapes of these arsenoriboses were improved allowing lower amounts to be quantified. The quantification of OH-ribose and PO4-ribose, however, was sample dependent and not possible if arsenobetaine (AB) and (DMA) were present at large concentrations in samples because of coelution of these species with OH-ribose and PO4-ribose, respectively. In the absence of OH-ribose, AB can be determined using the zirconium column while AB on the PRP-X100 column coelutes with arsenic cations.
Original language | English |
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Article number | 104099 |
Pages (from-to) | 1-11 |
Number of pages | 11 |
Journal | Microchemical Journal |
Volume | 150 |
DOIs | |
Publication status | Published - Nov 2019 |