The ZW Micro-Sex Chromosomes of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis, Trionychidae, Testudines) Have the Same Origin as Chicken Chromosome 15

T Kawagoshi, Yoshinobu Uno, Kazumi Matsubara, Yoichi Matsuda, Chizuko Nishida

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The Chinese soft-shelled turtle (Pelodiscus sinensis, Trionychidae, Testudines) has ZZ/ZW-type micro-sex chromosomes where the 18S-28S ribosomal RNA genes (18S-28S rDNA) are located. The W chromosome is morphologically differentiated from the Z chromosome by partial deletion and amplification of 18S-28S rDNA and W-specific repetitive sequences. We recently found a functional gene (TOP3B) mapped on the P. sinensis Z chromosome, which is located on chicken (Gallus gallus, GGA) chromosome 15. Then we cloned turtle homologues of 4 other GGA15-linked genes (GIT2, NF2, SBNO1, SF3A1) and localized them to P. sinensis chromosomes. The 4 genes all mapped on the Z chromosome, and 2 of them (SBNO1, SF3A1) were also localized to the W chromosome. Our mapping data suggest that at least one large inversion occurred between GGA15 and the P. sinensis Z chromosome, and that there are homologous regions in the distal portions of both the short and long arms between the Z and W chromosomes. W chromosomal differentiation in P. sinensis probably proceeded by the deletion of the proximal chromosomal region followed by 18S-28S rDNA amplification, after a paracentric inversion occurred at the breakpoints between the distal region of 18S-28S rDNA and the proximal region of SBNO1 on the Z chromosome.
Original languageEnglish
Pages (from-to)125-131
Number of pages7
JournalCytogenetic and Genome Research
Volume125
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 15
Sex Chromosomes
Turtles
Chickens
Chromosomes
Ribosomal DNA
28S Ribosomal RNA
18S Ribosomal RNA
Genes
Chromosome Deletion
Chromosomes, Human, Pair 2
Nucleic Acid Repetitive Sequences
rRNA Genes

Cite this

Kawagoshi, T ; Uno, Yoshinobu ; Matsubara, Kazumi ; Matsuda, Yoichi ; Nishida, Chizuko. / The ZW Micro-Sex Chromosomes of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis, Trionychidae, Testudines) Have the Same Origin as Chicken Chromosome 15. In: Cytogenetic and Genome Research. 2009 ; Vol. 125. pp. 125-131.
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abstract = "The Chinese soft-shelled turtle (Pelodiscus sinensis, Trionychidae, Testudines) has ZZ/ZW-type micro-sex chromosomes where the 18S-28S ribosomal RNA genes (18S-28S rDNA) are located. The W chromosome is morphologically differentiated from the Z chromosome by partial deletion and amplification of 18S-28S rDNA and W-specific repetitive sequences. We recently found a functional gene (TOP3B) mapped on the P. sinensis Z chromosome, which is located on chicken (Gallus gallus, GGA) chromosome 15. Then we cloned turtle homologues of 4 other GGA15-linked genes (GIT2, NF2, SBNO1, SF3A1) and localized them to P. sinensis chromosomes. The 4 genes all mapped on the Z chromosome, and 2 of them (SBNO1, SF3A1) were also localized to the W chromosome. Our mapping data suggest that at least one large inversion occurred between GGA15 and the P. sinensis Z chromosome, and that there are homologous regions in the distal portions of both the short and long arms between the Z and W chromosomes. W chromosomal differentiation in P. sinensis probably proceeded by the deletion of the proximal chromosomal region followed by 18S-28S rDNA amplification, after a paracentric inversion occurred at the breakpoints between the distal region of 18S-28S rDNA and the proximal region of SBNO1 on the Z chromosome.",
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The ZW Micro-Sex Chromosomes of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis, Trionychidae, Testudines) Have the Same Origin as Chicken Chromosome 15. / Kawagoshi, T; Uno, Yoshinobu; Matsubara, Kazumi; Matsuda, Yoichi; Nishida, Chizuko.

In: Cytogenetic and Genome Research, Vol. 125, 2009, p. 125-131.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The ZW Micro-Sex Chromosomes of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis, Trionychidae, Testudines) Have the Same Origin as Chicken Chromosome 15

AU - Kawagoshi, T

AU - Uno, Yoshinobu

AU - Matsubara, Kazumi

AU - Matsuda, Yoichi

AU - Nishida, Chizuko

PY - 2009

Y1 - 2009

N2 - The Chinese soft-shelled turtle (Pelodiscus sinensis, Trionychidae, Testudines) has ZZ/ZW-type micro-sex chromosomes where the 18S-28S ribosomal RNA genes (18S-28S rDNA) are located. The W chromosome is morphologically differentiated from the Z chromosome by partial deletion and amplification of 18S-28S rDNA and W-specific repetitive sequences. We recently found a functional gene (TOP3B) mapped on the P. sinensis Z chromosome, which is located on chicken (Gallus gallus, GGA) chromosome 15. Then we cloned turtle homologues of 4 other GGA15-linked genes (GIT2, NF2, SBNO1, SF3A1) and localized them to P. sinensis chromosomes. The 4 genes all mapped on the Z chromosome, and 2 of them (SBNO1, SF3A1) were also localized to the W chromosome. Our mapping data suggest that at least one large inversion occurred between GGA15 and the P. sinensis Z chromosome, and that there are homologous regions in the distal portions of both the short and long arms between the Z and W chromosomes. W chromosomal differentiation in P. sinensis probably proceeded by the deletion of the proximal chromosomal region followed by 18S-28S rDNA amplification, after a paracentric inversion occurred at the breakpoints between the distal region of 18S-28S rDNA and the proximal region of SBNO1 on the Z chromosome.

AB - The Chinese soft-shelled turtle (Pelodiscus sinensis, Trionychidae, Testudines) has ZZ/ZW-type micro-sex chromosomes where the 18S-28S ribosomal RNA genes (18S-28S rDNA) are located. The W chromosome is morphologically differentiated from the Z chromosome by partial deletion and amplification of 18S-28S rDNA and W-specific repetitive sequences. We recently found a functional gene (TOP3B) mapped on the P. sinensis Z chromosome, which is located on chicken (Gallus gallus, GGA) chromosome 15. Then we cloned turtle homologues of 4 other GGA15-linked genes (GIT2, NF2, SBNO1, SF3A1) and localized them to P. sinensis chromosomes. The 4 genes all mapped on the Z chromosome, and 2 of them (SBNO1, SF3A1) were also localized to the W chromosome. Our mapping data suggest that at least one large inversion occurred between GGA15 and the P. sinensis Z chromosome, and that there are homologous regions in the distal portions of both the short and long arms between the Z and W chromosomes. W chromosomal differentiation in P. sinensis probably proceeded by the deletion of the proximal chromosomal region followed by 18S-28S rDNA amplification, after a paracentric inversion occurred at the breakpoints between the distal region of 18S-28S rDNA and the proximal region of SBNO1 on the Z chromosome.

KW - Chicken

KW - Chromosome homology

KW - Chromosome rearrangement

KW - Comparative mapping

KW - FISH

KW - Reptile

KW - Sex chromosome

KW - Sex determination

KW - Turtle

KW - ZW.

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M3 - Article

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SP - 125

EP - 131

JO - Cytogenetics and Cell Genetics

JF - Cytogenetics and Cell Genetics

SN - 0301-0171

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