Thio arsenic species measurements in marine organisms and geothermal waters

Bill MAHER, Simon FOSTER, K Hug, J Moreau

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Recently two new classes of arsenic species have been identified, thio-methylated arsenic species in marine organisms where the oxygen bonded to arsenic is replaced by an S group and thioarsenate species in sulfide rich water environments. Here we describe the use of HPLC-ICPMS to measure thio arsenic species found in marine biota and a geothermal water sample from a sulfide rich environment. Thio-methylated arsenic species were separated using an Alantis C18 reverse phase column and elution with an aqueous 20mM phosphate buffer (pH3). Thioarsenate species were separated using an Ion Pac, AS16 anion exchange column with a sodium hydroxide gradient (20–100 mM) and the use of an anionic self-regenerating suppressor to remove sodium ions before the ICPMS spray chamber. Thio-methylated arsenic species in marine biota are stable to freeze drying and microwave extraction with methanol–water (1:1 v/v) at 70 °C. Rotational mixing at 25 °C for long periods causes the loss of species. Freeze–thawing of extracts results in oxidation of species. Thio-methylated arsenic species in extracts are stable if the solvent is removed and the residues stored dry in a dessicator. Thioarsenate species in water samples are stable during analysis if manipulated under a nitrogen atmosphere and if during chromatography mobile phases are degassed and precautions taken to exclude air from samples.
    Original languageEnglish
    Pages (from-to)82-90
    Number of pages9
    JournalMicrochemical Journal
    Volume111
    DOIs
    Publication statusPublished - 2013

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    Arsenic
    Water
    Sulfides
    Ions
    Sodium Hydroxide
    Chromatography
    Anions
    Drying
    Buffers
    Nitrogen
    Sodium
    Phosphates
    Microwaves
    Oxygen
    Oxidation
    Air

    Cite this

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    title = "Thio arsenic species measurements in marine organisms and geothermal waters",
    abstract = "Recently two new classes of arsenic species have been identified, thio-methylated arsenic species in marine organisms where the oxygen bonded to arsenic is replaced by an S group and thioarsenate species in sulfide rich water environments. Here we describe the use of HPLC-ICPMS to measure thio arsenic species found in marine biota and a geothermal water sample from a sulfide rich environment. Thio-methylated arsenic species were separated using an Alantis C18 reverse phase column and elution with an aqueous 20mM phosphate buffer (pH3). Thioarsenate species were separated using an Ion Pac, AS16 anion exchange column with a sodium hydroxide gradient (20–100 mM) and the use of an anionic self-regenerating suppressor to remove sodium ions before the ICPMS spray chamber. Thio-methylated arsenic species in marine biota are stable to freeze drying and microwave extraction with methanol–water (1:1 v/v) at 70 °C. Rotational mixing at 25 °C for long periods causes the loss of species. Freeze–thawing of extracts results in oxidation of species. Thio-methylated arsenic species in extracts are stable if the solvent is removed and the residues stored dry in a dessicator. Thioarsenate species in water samples are stable during analysis if manipulated under a nitrogen atmosphere and if during chromatography mobile phases are degassed and precautions taken to exclude air from samples.",
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    author = "Bill MAHER and Simon FOSTER and K Hug and J Moreau",
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    Thio arsenic species measurements in marine organisms and geothermal waters. / MAHER, Bill; FOSTER, Simon; Hug, K; Moreau, J.

    In: Microchemical Journal, Vol. 111, 2013, p. 82-90.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Thio arsenic species measurements in marine organisms and geothermal waters

    AU - MAHER, Bill

    AU - FOSTER, Simon

    AU - Hug, K

    AU - Moreau, J

    PY - 2013

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    AB - Recently two new classes of arsenic species have been identified, thio-methylated arsenic species in marine organisms where the oxygen bonded to arsenic is replaced by an S group and thioarsenate species in sulfide rich water environments. Here we describe the use of HPLC-ICPMS to measure thio arsenic species found in marine biota and a geothermal water sample from a sulfide rich environment. Thio-methylated arsenic species were separated using an Alantis C18 reverse phase column and elution with an aqueous 20mM phosphate buffer (pH3). Thioarsenate species were separated using an Ion Pac, AS16 anion exchange column with a sodium hydroxide gradient (20–100 mM) and the use of an anionic self-regenerating suppressor to remove sodium ions before the ICPMS spray chamber. Thio-methylated arsenic species in marine biota are stable to freeze drying and microwave extraction with methanol–water (1:1 v/v) at 70 °C. Rotational mixing at 25 °C for long periods causes the loss of species. Freeze–thawing of extracts results in oxidation of species. Thio-methylated arsenic species in extracts are stable if the solvent is removed and the residues stored dry in a dessicator. Thioarsenate species in water samples are stable during analysis if manipulated under a nitrogen atmosphere and if during chromatography mobile phases are degassed and precautions taken to exclude air from samples.

    KW - Arsenic

    KW - Thioarsenic species

    KW - HPLC_ICPMS

    KW - Marine organisms

    KW - Geothermal waters.

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    DO - 10.1016/j.microc.2012.12.008

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