Thyroid hormone modulates offspring sex ratio in a turtle with temperature-dependent sex determination

B. J Sun, T Li, Y Mu, J McGlashan, Arthur GEORGES, R Shine, W-G Du

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The adaptive significance of temperature-dependent sex determination (TSD) has attracted a great deal of research, but the underlying mechanisms by which temperature determines the sex of a developing embryo remain poorly understood. Here, we manipulated the level of a thyroid hormone (TH), triiodothyronine (T3), during embryonic development (by adding excess T3 to the eggs of the red-eared slider turtle Trachemys scripta, a reptile with TSD), to test two competing hypotheses on the proximate basis for TSD: the developmental rate hypothesis versus the hormone hypothesis Exogenous TH accelerated embryonic heart rate (and hence metabolic rate), developmental rate, and rates of early post-hatching growth. More importantly, hyperthyroid conditions depressed expression of Cyp19a1 (the gene encoding for aromatase) and levels of oestradiol, and induced more male offspring. This result is contrary to the direction of sex-ratio shift predicted by the developmental rate hypothesis, but consistent with that predicted by the hormone hypothesis Our results suggest an important role for THs in regulating sex steroid hormones, and therefore, in affecting gonadal sex differentiation in TSD reptiles. Our study has implications for the conservation of TSD reptiles in the context of global change because environmental contaminants may disrupt the activity of THs, and thereby affect offspring sex in TSD reptiles.

    Original languageEnglish
    Pages (from-to)1-8
    Number of pages8
    JournalProceedings of the Royal Society B: Biological Sciences
    Volume283
    Issue number1841
    DOIs
    Publication statusPublished - 26 Oct 2016

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    sex determination
    Turtles
    Sex Ratio
    thyroid hormones
    Thyroid Hormones
    turtle
    turtles
    sex ratio
    hormone
    Temperature
    Reptiles
    gender
    reptile
    reptiles
    temperature
    triiodothyronine
    Triiodothyronine
    Hormones
    hormones
    Gene encoding

    Cite this

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    title = "Thyroid hormone modulates offspring sex ratio in a turtle with temperature-dependent sex determination",
    abstract = "The adaptive significance of temperature-dependent sex determination (TSD) has attracted a great deal of research, but the underlying mechanisms by which temperature determines the sex of a developing embryo remain poorly understood. Here, we manipulated the level of a thyroid hormone (TH), triiodothyronine (T3), during embryonic development (by adding excess T3 to the eggs of the red-eared slider turtle Trachemys scripta, a reptile with TSD), to test two competing hypotheses on the proximate basis for TSD: the developmental rate hypothesis versus the hormone hypothesis Exogenous TH accelerated embryonic heart rate (and hence metabolic rate), developmental rate, and rates of early post-hatching growth. More importantly, hyperthyroid conditions depressed expression of Cyp19a1 (the gene encoding for aromatase) and levels of oestradiol, and induced more male offspring. This result is contrary to the direction of sex-ratio shift predicted by the developmental rate hypothesis, but consistent with that predicted by the hormone hypothesis Our results suggest an important role for THs in regulating sex steroid hormones, and therefore, in affecting gonadal sex differentiation in TSD reptiles. Our study has implications for the conservation of TSD reptiles in the context of global change because environmental contaminants may disrupt the activity of THs, and thereby affect offspring sex in TSD reptiles.",
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    Thyroid hormone modulates offspring sex ratio in a turtle with temperature-dependent sex determination. / Sun, B. J; Li, T; Mu, Y; McGlashan, J; GEORGES, Arthur; Shine, R; Du, W-G.

    In: Proceedings of the Royal Society B: Biological Sciences, Vol. 283, No. 1841, 26.10.2016, p. 1-8.

    Research output: Contribution to journalArticle

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    AU - Li, T

    AU - Mu, Y

    AU - McGlashan, J

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    AU - Shine, R

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