Tissue turnover in ovine muscles and lipids as recorded by multiple (H, C, O, S) stable isotope ratios

Sabine M. Harrison, Olafz Schmidt, Aidan P. Moloney, Simon D. Kelly, Andreas Rossmann, Antje Schellenberg, Federica Camin, Matteo Perini, Jurian Hoogewerff, Frank J. Monahan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Multiple stable isotope ratios (δ2H, δ13C, δ18O and δ34S) were measured in muscle, muscle lipids and lipid fractions collected from 28 lambs, subjected to a diet-switch and raised on two energy allowances (EAs), to determine tissue turnover and diet-tissue fractionation. The diet-muscle fractionations prior to the diet-switch were estimated to be -44.0‰, +1.9‰ and 0‰ for H, C and S, respectively, while the drinking water was demonstrated to be the main source of muscle O and thus δ18O variation. The diet-intra-muscular lipid fractionations prior to the diet-switch were estimated to be -172.7‰, -1.3‰ and -11.5‰ for H, C and O, respectively. The C half-lives of muscle were determined to be 75.7 and 91.6days for animals receiving the high and low EA, respectively. Extracting temporally resolved pre-slaughter dietary information from meat by analysing bulk muscle, muscle lipids and muscle lipid fractions appeared to be not practicable due to possible incomplete turnover of lipids.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalFood Chemistry
Volume124
Issue number1
DOIs
Publication statusPublished - 1 Jan 2011
Externally publishedYes

Fingerprint

Isotopes
stable isotopes
Muscle
Sheep
Nutrition
Tissue
Lipids
sheep
Muscles
muscles
lipids
Diet
Fractionation
diet
fractionation
Switches
tissues
Meats
energy
Drinking Water

Cite this

Harrison, S. M., Schmidt, O., Moloney, A. P., Kelly, S. D., Rossmann, A., Schellenberg, A., ... Monahan, F. J. (2011). Tissue turnover in ovine muscles and lipids as recorded by multiple (H, C, O, S) stable isotope ratios. Food Chemistry, 124(1), 291-297. https://doi.org/10.1016/j.foodchem.2010.06.035
Harrison, Sabine M. ; Schmidt, Olafz ; Moloney, Aidan P. ; Kelly, Simon D. ; Rossmann, Andreas ; Schellenberg, Antje ; Camin, Federica ; Perini, Matteo ; Hoogewerff, Jurian ; Monahan, Frank J. / Tissue turnover in ovine muscles and lipids as recorded by multiple (H, C, O, S) stable isotope ratios. In: Food Chemistry. 2011 ; Vol. 124, No. 1. pp. 291-297.
@article{77978eb2f6204a2dafe93e195b3fd34d,
title = "Tissue turnover in ovine muscles and lipids as recorded by multiple (H, C, O, S) stable isotope ratios",
abstract = "Multiple stable isotope ratios (δ2H, δ13C, δ18O and δ34S) were measured in muscle, muscle lipids and lipid fractions collected from 28 lambs, subjected to a diet-switch and raised on two energy allowances (EAs), to determine tissue turnover and diet-tissue fractionation. The diet-muscle fractionations prior to the diet-switch were estimated to be -44.0‰, +1.9‰ and 0‰ for H, C and S, respectively, while the drinking water was demonstrated to be the main source of muscle O and thus δ18O variation. The diet-intra-muscular lipid fractionations prior to the diet-switch were estimated to be -172.7‰, -1.3‰ and -11.5‰ for H, C and O, respectively. The C half-lives of muscle were determined to be 75.7 and 91.6days for animals receiving the high and low EA, respectively. Extracting temporally resolved pre-slaughter dietary information from meat by analysing bulk muscle, muscle lipids and muscle lipid fractions appeared to be not practicable due to possible incomplete turnover of lipids.",
keywords = "Carbon, Hydrogen, IRMS, Isotopic clock, Lamb, Lipid, Meat, Muscle, Oxygen, Stable isotopes, Sulphur",
author = "Harrison, {Sabine M.} and Olafz Schmidt and Moloney, {Aidan P.} and Kelly, {Simon D.} and Andreas Rossmann and Antje Schellenberg and Federica Camin and Matteo Perini and Jurian Hoogewerff and Monahan, {Frank J.}",
year = "2011",
month = "1",
day = "1",
doi = "10.1016/j.foodchem.2010.06.035",
language = "English",
volume = "124",
pages = "291--297",
journal = "Journal of Micronutrient Analysis",
issn = "0308-8146",
publisher = "Elsevier Limited",
number = "1",

}

Harrison, SM, Schmidt, O, Moloney, AP, Kelly, SD, Rossmann, A, Schellenberg, A, Camin, F, Perini, M, Hoogewerff, J & Monahan, FJ 2011, 'Tissue turnover in ovine muscles and lipids as recorded by multiple (H, C, O, S) stable isotope ratios', Food Chemistry, vol. 124, no. 1, pp. 291-297. https://doi.org/10.1016/j.foodchem.2010.06.035

Tissue turnover in ovine muscles and lipids as recorded by multiple (H, C, O, S) stable isotope ratios. / Harrison, Sabine M.; Schmidt, Olafz; Moloney, Aidan P.; Kelly, Simon D.; Rossmann, Andreas; Schellenberg, Antje; Camin, Federica; Perini, Matteo; Hoogewerff, Jurian; Monahan, Frank J.

In: Food Chemistry, Vol. 124, No. 1, 01.01.2011, p. 291-297.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tissue turnover in ovine muscles and lipids as recorded by multiple (H, C, O, S) stable isotope ratios

AU - Harrison, Sabine M.

AU - Schmidt, Olafz

AU - Moloney, Aidan P.

AU - Kelly, Simon D.

AU - Rossmann, Andreas

AU - Schellenberg, Antje

AU - Camin, Federica

AU - Perini, Matteo

AU - Hoogewerff, Jurian

AU - Monahan, Frank J.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Multiple stable isotope ratios (δ2H, δ13C, δ18O and δ34S) were measured in muscle, muscle lipids and lipid fractions collected from 28 lambs, subjected to a diet-switch and raised on two energy allowances (EAs), to determine tissue turnover and diet-tissue fractionation. The diet-muscle fractionations prior to the diet-switch were estimated to be -44.0‰, +1.9‰ and 0‰ for H, C and S, respectively, while the drinking water was demonstrated to be the main source of muscle O and thus δ18O variation. The diet-intra-muscular lipid fractionations prior to the diet-switch were estimated to be -172.7‰, -1.3‰ and -11.5‰ for H, C and O, respectively. The C half-lives of muscle were determined to be 75.7 and 91.6days for animals receiving the high and low EA, respectively. Extracting temporally resolved pre-slaughter dietary information from meat by analysing bulk muscle, muscle lipids and muscle lipid fractions appeared to be not practicable due to possible incomplete turnover of lipids.

AB - Multiple stable isotope ratios (δ2H, δ13C, δ18O and δ34S) were measured in muscle, muscle lipids and lipid fractions collected from 28 lambs, subjected to a diet-switch and raised on two energy allowances (EAs), to determine tissue turnover and diet-tissue fractionation. The diet-muscle fractionations prior to the diet-switch were estimated to be -44.0‰, +1.9‰ and 0‰ for H, C and S, respectively, while the drinking water was demonstrated to be the main source of muscle O and thus δ18O variation. The diet-intra-muscular lipid fractionations prior to the diet-switch were estimated to be -172.7‰, -1.3‰ and -11.5‰ for H, C and O, respectively. The C half-lives of muscle were determined to be 75.7 and 91.6days for animals receiving the high and low EA, respectively. Extracting temporally resolved pre-slaughter dietary information from meat by analysing bulk muscle, muscle lipids and muscle lipid fractions appeared to be not practicable due to possible incomplete turnover of lipids.

KW - Carbon

KW - Hydrogen

KW - IRMS

KW - Isotopic clock

KW - Lamb

KW - Lipid

KW - Meat

KW - Muscle

KW - Oxygen

KW - Stable isotopes

KW - Sulphur

UR - http://www.scopus.com/inward/record.url?scp=77955272632&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2010.06.035

DO - 10.1016/j.foodchem.2010.06.035

M3 - Article

VL - 124

SP - 291

EP - 297

JO - Journal of Micronutrient Analysis

JF - Journal of Micronutrient Analysis

SN - 0308-8146

IS - 1

ER -

Harrison SM, Schmidt O, Moloney AP, Kelly SD, Rossmann A, Schellenberg A et al. Tissue turnover in ovine muscles and lipids as recorded by multiple (H, C, O, S) stable isotope ratios. Food Chemistry. 2011 Jan 1;124(1):291-297. https://doi.org/10.1016/j.foodchem.2010.06.035