TY - JOUR
T1 - Tob1 is expressed in developing and adult gonads and is associated with the P-body marker, Dcp2
AU - Shapouri, Farnaz
AU - Saeidi, Shaghayegh
AU - de Iongh, Robb U.
AU - Casagranda, Franca
AU - Western, Patrick S.
AU - McLaughlin, Eileen A.
AU - Sutherland, Jessie M.
AU - Hime, Gary R.
AU - Familari, Mary
N1 - Funding Information:
These studies were supported by funds provided to M.F. by the School of Biosciences, University of Melbourne.
Publisher Copyright:
© 2015, Springer-Verlag Berlin Heidelberg.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Tob1 is a member of the BTG/TOB family of proteins with established antiproliferative function. In Danio rerio and Xenopus laevis, the Tob1 gene is expressed from the one-cell stage through to early gastrula stages, followed in later development by discrete expression in many tissues including the notochord and somites. In both mouse and human, Tob1 is expressed in many adult tissues including the testis and ovary; however, the specific cell types are unknown. We examine Tob1 gene expression in mouse in developing germ cells and in sorted male germ cells (gonocytes, spermatogonia, pachytene spermatocytes and round spermatids) by reverse transcription and droplet digital polymerase chain reaction (RT-ddPCR) and in adult ovary and testis by immunofluorescence with anti-Tob1 protein staining. By RT-ddPCR, Tob1 expression was low in developing male germ cells but was highly expressed in round spermatids. In developing female germ cells undergoing entry into meiosis, it increased 10-fold. Tob1 was also highly expressed in round spermatids and in oocytes in all stages of folliculogenesis. Notably, a marker for P-bodies, Dcp-2, was also highly expressed in round spermatids and all oocyte stages examined. The cytoplasmic presence of Tob1 protein in round spermatids and oocytes and the association of Tob1 protein with Dcp2 in both cell types suggest that Tob1 protein plays a role in post-transcriptional mechanisms.
AB - Tob1 is a member of the BTG/TOB family of proteins with established antiproliferative function. In Danio rerio and Xenopus laevis, the Tob1 gene is expressed from the one-cell stage through to early gastrula stages, followed in later development by discrete expression in many tissues including the notochord and somites. In both mouse and human, Tob1 is expressed in many adult tissues including the testis and ovary; however, the specific cell types are unknown. We examine Tob1 gene expression in mouse in developing germ cells and in sorted male germ cells (gonocytes, spermatogonia, pachytene spermatocytes and round spermatids) by reverse transcription and droplet digital polymerase chain reaction (RT-ddPCR) and in adult ovary and testis by immunofluorescence with anti-Tob1 protein staining. By RT-ddPCR, Tob1 expression was low in developing male germ cells but was highly expressed in round spermatids. In developing female germ cells undergoing entry into meiosis, it increased 10-fold. Tob1 was also highly expressed in round spermatids and in oocytes in all stages of folliculogenesis. Notably, a marker for P-bodies, Dcp-2, was also highly expressed in round spermatids and all oocyte stages examined. The cytoplasmic presence of Tob1 protein in round spermatids and oocytes and the association of Tob1 protein with Dcp2 in both cell types suggest that Tob1 protein plays a role in post-transcriptional mechanisms.
KW - Dcp2
KW - Droplet digital PCR
KW - Germ cells
KW - P-bodies
KW - Tob1
UR - http://www.scopus.com/inward/record.url?scp=84949685856&partnerID=8YFLogxK
U2 - 10.1007/s00441-015-2328-z
DO - 10.1007/s00441-015-2328-z
M3 - Article
C2 - 26662055
AN - SCOPUS:84949685856
SN - 0302-766X
VL - 364
SP - 443
EP - 451
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 2
ER -