Towards an effective strategy for extended storage of contact lenses in hotter climates

A challenge bacterial suspension of pseudomonas aeruginosa of 8 log cfu/ml was inoculated into contact lens cases containing Solocare Soft All-in-one Solution and a Focus monthly contact lens and stored for intervals of 10 minutes, 1 day, 7 days, 21 days, 28 days under 4, 24 and 37 degrees C constant temperature conditions designed to simulate temperatures below, and above 'room temperature'. At each of the intervals, samples from a case were taken, serially diluted, plated and incubated at 37 degrees of 24 hours, colony count performed to obtain cfu/ml of surviving bacteria at that time interval. The resultant data were analyzed in detail.