Tracking transparent monogenean parasites on fish from infection to maturity

Alejandro Trujillo-González, Constantin C. Constantinoiu, Richard Rowe, Kate S. Hutson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The infection dynamics and distribution of the ectoparasitic fish monogenean Neobenedenia sp. (Monogenea: Capsalidae) throughout its development was examined on barramundi, Lates calcarifer (Bloch) (Latidae), by labelling transparent, ciliated larvae (oncomiracidia) with a fluorescent dye. Replicate fish were each exposed to approximately 50 fluorescent oncomiracidia and then examined for parasites using an epifluorescence stereomicroscope at 10 time intervals post-exposure (15, 30, 60, 120min, 24, 48h, four, eight, 12, and 16 days). Fluorescent labelling revealed that parasites attached underneath and on the surface of the scales of host fish. Parasite infection success was 20% within 15min, and peaked at 93% two days post-exposure, before gradually declining between four and sixteen days. Differences in parasite distribution on L.calcarifer over time provided strong evidence that Neobenedenia sp. larvae settled opportunistically and then migrated to specific microhabitats. Parasites initially attached (<24h) in greater mean numbers on the body surface (13±1.5) compared to the fins (4±0.42) and head region (2±0.41). Once larvae recruitment had ceased (48h), there were significantly higher mean post-larvae counts on the head (5±3.4) and fins (12±3) compared to previous time intervals. Neobenedenia sp. aggregated on the eyes, fins, and dorsal and ventral extremities on the main body. As parasites neared sexual maturity, there was a marked aggregation on the fins (22±2.35) compared to the head (4±0.97) and body (9±1.33), indicating that Neobenedenia sp. may form mating aggregations.

Original languageEnglish
Pages (from-to)316-322
Number of pages7
JournalInternational Journal For Parasitology: Parasites And Wildlife
Volume4
Issue number3
DOIs
Publication statusPublished - Dec 2015
Externally publishedYes

Fingerprint

Fishes
Parasites
Larva
parasites
fins
Infection
fish
infection
Head
Lates calcarifer
larvae
Latidae
Capsalidae
Parasitic Diseases
fluorescent labeling
Monogenea
Fluorescent Dyes
fluorescent dyes
sexual maturity
Extremities

Cite this

Trujillo-González, Alejandro ; Constantinoiu, Constantin C. ; Rowe, Richard ; Hutson, Kate S. / Tracking transparent monogenean parasites on fish from infection to maturity. In: International Journal For Parasitology: Parasites And Wildlife. 2015 ; Vol. 4, No. 3. pp. 316-322.
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Tracking transparent monogenean parasites on fish from infection to maturity. / Trujillo-González, Alejandro; Constantinoiu, Constantin C.; Rowe, Richard; Hutson, Kate S.

In: International Journal For Parasitology: Parasites And Wildlife, Vol. 4, No. 3, 12.2015, p. 316-322.

Research output: Contribution to journalArticle

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