Trimethyl-β-cyclodextrin-encapsulated monolithic capillary columns: Preparation, characterization and chiral nano-LC application

Ashraf Ghanem, Frady G. Adly, Yasser Sokerik, Nana Yaa Antwi, Mohamed A. Shenashen, Sherif A. El-Safty

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Trimethylated-β-cyclodextrin (TM-β-CD) was encapsulated within several polymer monolithic capillary columns for reversed-phase chiral nano-liquid chromatography (nano-LC). The monolithic phases were prepared using the one-pot in situ copolymerization of ethylene glycol dimethacrylate (EDMA), glycidyl methacrylate (GMA) monomers and 1-propanol, 1,4-butanediol as progenic solvents in presence of TM-β-CD solution within fused silica capillaries (150 µm I.D.). The obtained chiral monolithic stationery phases were characterized by scanning electron microscopy (SEM), N2 adsorption/desorption isotherms, wide angle x-ray diffraction (WAXRD), thermogravimetric analysis (TGA) and differential thermal analysis (DTA). The materials characterization demonstrated that monolithic phases with higher concentration of TM-β-CD have relatively larger surface area, smaller pore size and larger total pore volume compared to those with lower concentration TM-β-CD. The prepared columns were tested for their enantioseparation efficiency of a range of racemic pharmaceuticals. The screening results demonstrated the potential of functionalizing polymer monolithic stationary phases with TM-β-CD using the in situ encapsulation approach

Original languageEnglish
Pages (from-to)239-248
Number of pages10
JournalTalanta
Volume169
DOIs
Publication statusPublished - 1 Jul 2017

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Liquid chromatography
Cyclodextrins
Polymers
1-Propanol
Fused silica
Encapsulation
Differential thermal analysis
Copolymerization
Pore size
Isotherms
Thermogravimetric analysis
Desorption
Screening
Diffraction
Monomers
Adsorption
X rays
Scanning electron microscopy
Pharmaceutical Preparations

Cite this

Ghanem, Ashraf ; Adly, Frady G. ; Sokerik, Yasser ; Antwi, Nana Yaa ; Shenashen, Mohamed A. ; El-Safty, Sherif A. / Trimethyl-β-cyclodextrin-encapsulated monolithic capillary columns: Preparation, characterization and chiral nano-LC application. In: Talanta. 2017 ; Vol. 169. pp. 239-248.
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abstract = "Trimethylated-β-cyclodextrin (TM-β-CD) was encapsulated within several polymer monolithic capillary columns for reversed-phase chiral nano-liquid chromatography (nano-LC). The monolithic phases were prepared using the one-pot in situ copolymerization of ethylene glycol dimethacrylate (EDMA), glycidyl methacrylate (GMA) monomers and 1-propanol, 1,4-butanediol as progenic solvents in presence of TM-β-CD solution within fused silica capillaries (150 µm I.D.). The obtained chiral monolithic stationery phases were characterized by scanning electron microscopy (SEM), N2 adsorption/desorption isotherms, wide angle x-ray diffraction (WAXRD), thermogravimetric analysis (TGA) and differential thermal analysis (DTA). The materials characterization demonstrated that monolithic phases with higher concentration of TM-β-CD have relatively larger surface area, smaller pore size and larger total pore volume compared to those with lower concentration TM-β-CD. The prepared columns were tested for their enantioseparation efficiency of a range of racemic pharmaceuticals. The screening results demonstrated the potential of functionalizing polymer monolithic stationary phases with TM-β-CD using the in situ encapsulation approach",
keywords = "Chiral resolution, CLC, Cyclodextrin, Enantioseparation, Monolith, Nano-LC",
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Trimethyl-β-cyclodextrin-encapsulated monolithic capillary columns: Preparation, characterization and chiral nano-LC application. / Ghanem, Ashraf; Adly, Frady G.; Sokerik, Yasser; Antwi, Nana Yaa; Shenashen, Mohamed A.; El-Safty, Sherif A.

In: Talanta, Vol. 169, 01.07.2017, p. 239-248.

Research output: Contribution to journalArticle

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T1 - Trimethyl-β-cyclodextrin-encapsulated monolithic capillary columns: Preparation, characterization and chiral nano-LC application

AU - Ghanem, Ashraf

AU - Adly, Frady G.

AU - Sokerik, Yasser

AU - Antwi, Nana Yaa

AU - Shenashen, Mohamed A.

AU - El-Safty, Sherif A.

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AB - Trimethylated-β-cyclodextrin (TM-β-CD) was encapsulated within several polymer monolithic capillary columns for reversed-phase chiral nano-liquid chromatography (nano-LC). The monolithic phases were prepared using the one-pot in situ copolymerization of ethylene glycol dimethacrylate (EDMA), glycidyl methacrylate (GMA) monomers and 1-propanol, 1,4-butanediol as progenic solvents in presence of TM-β-CD solution within fused silica capillaries (150 µm I.D.). The obtained chiral monolithic stationery phases were characterized by scanning electron microscopy (SEM), N2 adsorption/desorption isotherms, wide angle x-ray diffraction (WAXRD), thermogravimetric analysis (TGA) and differential thermal analysis (DTA). The materials characterization demonstrated that monolithic phases with higher concentration of TM-β-CD have relatively larger surface area, smaller pore size and larger total pore volume compared to those with lower concentration TM-β-CD. The prepared columns were tested for their enantioseparation efficiency of a range of racemic pharmaceuticals. The screening results demonstrated the potential of functionalizing polymer monolithic stationary phases with TM-β-CD using the in situ encapsulation approach

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KW - CLC

KW - Cyclodextrin

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