Trimethyl-β-cyclodextrin-encapsulated monolithic capillary columns: Preparation, characterization and chiral nano-LC application

Ashraf Ghanem, Frady G. Adly, Yasser Sokerik, Nana Yaa Antwi, Mohamed A. Shenashen, Sherif A. El-Safty

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Trimethylated-β-cyclodextrin (TM-β-CD) was encapsulated within several polymer monolithic capillary columns for reversed-phase chiral nano-liquid chromatography (nano-LC). The monolithic phases were prepared using the one-pot in situ copolymerization of ethylene glycol dimethacrylate (EDMA), glycidyl methacrylate (GMA) monomers and 1-propanol, 1,4-butanediol as progenic solvents in presence of TM-β-CD solution within fused silica capillaries (150 µm I.D.). The obtained chiral monolithic stationery phases were characterized by scanning electron microscopy (SEM), N2 adsorption/desorption isotherms, wide angle x-ray diffraction (WAXRD), thermogravimetric analysis (TGA) and differential thermal analysis (DTA). The materials characterization demonstrated that monolithic phases with higher concentration of TM-β-CD have relatively larger surface area, smaller pore size and larger total pore volume compared to those with lower concentration TM-β-CD. The prepared columns were tested for their enantioseparation efficiency of a range of racemic pharmaceuticals. The screening results demonstrated the potential of functionalizing polymer monolithic stationary phases with TM-β-CD using the in situ encapsulation approach

Original languageEnglish
Pages (from-to)239-248
Number of pages10
JournalTalanta
Volume169
DOIs
Publication statusPublished - 1 Jul 2017

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