Two in one: cryptic species discovered in biological control agent populations using molecular data and crossbreeding experiments

Iain Paterson, Rosie Mangan, Douglas Downie, Julie Coetzee, Martin Hill, Ashley Burke, Paul DOWNEY, Thomas Henry, Stephe Compton

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    There are many examples of cryptic species that have been identified through DNA-barcoding or other genetic techniques. There are, however, very few confirmations of cryptic species being reproductively isolated. This study presents one of the few cases of cryptic species that has been confirmed to be reproductively isolated and therefore true species according to the biological species concept. The cryptic species are of special interest because they were discovered within biological control agent populations. Two geographically isolated populations of Eccritotarsus catarinensis (Carvalho) [Hemiptera: Miridae], a biological control agent for the invasive aquatic macrophyte, water hyacinth, Eichhornia crassipes (Mart.) Solms [Pontederiaceae], in South Africa, were sampled from the native range of the species in South America. Morphological characteristics indicated that both populations were the same species according to the current taxonomy, but subsequent DNA analysis and breeding experiments revealed that the two populations are reproductively isolated. Crossbreeding experiments resulted in very few hybrid offspring when individuals were forced to interbreed with individuals of the other population, and no hybrid offspring were recorded when a choice of mate from either population was offered. The data indicate that the two populations are cryptic species that are reproductively incompatible. Subtle but reliable diagnostic characteristics were then identified to distinguish between the two species which would have been considered intraspecific variation without the data from the genetics and interbreeding experiments. These findings suggest that all consignments of biological control agents from allopatric populations should be screened for cryptic species using genetic techniques and that the importation of multiple consignments of the same species for biological control should be conducted with caution.
    Original languageEnglish
    Pages (from-to)6139-6150
    Number of pages12
    JournalEcology and Evolution
    Volume6
    Issue number17
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    biological control
    biological control agents
    experiment
    Eichhornia crassipes
    Eccritotarsus catarinensis
    Pontederiaceae
    Miridae
    allopatry
    DNA barcoding
    mating behavior
    Hemiptera
    DNA
    species concept
    South Africa
    isolated population
    intraspecific variation
    taxonomy
    macrophyte
    breeding
    methodology

    Cite this

    Paterson, Iain ; Mangan, Rosie ; Downie, Douglas ; Coetzee, Julie ; Hill, Martin ; Burke, Ashley ; DOWNEY, Paul ; Henry, Thomas ; Compton, Stephe. / Two in one: cryptic species discovered in biological control agent populations using molecular data and crossbreeding experiments. In: Ecology and Evolution. 2016 ; Vol. 6, No. 17. pp. 6139-6150.
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    abstract = "There are many examples of cryptic species that have been identified through DNA-barcoding or other genetic techniques. There are, however, very few confirmations of cryptic species being reproductively isolated. This study presents one of the few cases of cryptic species that has been confirmed to be reproductively isolated and therefore true species according to the biological species concept. The cryptic species are of special interest because they were discovered within biological control agent populations. Two geographically isolated populations of Eccritotarsus catarinensis (Carvalho) [Hemiptera: Miridae], a biological control agent for the invasive aquatic macrophyte, water hyacinth, Eichhornia crassipes (Mart.) Solms [Pontederiaceae], in South Africa, were sampled from the native range of the species in South America. Morphological characteristics indicated that both populations were the same species according to the current taxonomy, but subsequent DNA analysis and breeding experiments revealed that the two populations are reproductively isolated. Crossbreeding experiments resulted in very few hybrid offspring when individuals were forced to interbreed with individuals of the other population, and no hybrid offspring were recorded when a choice of mate from either population was offered. The data indicate that the two populations are cryptic species that are reproductively incompatible. Subtle but reliable diagnostic characteristics were then identified to distinguish between the two species which would have been considered intraspecific variation without the data from the genetics and interbreeding experiments. These findings suggest that all consignments of biological control agents from allopatric populations should be screened for cryptic species using genetic techniques and that the importation of multiple consignments of the same species for biological control should be conducted with caution.",
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    author = "Iain Paterson and Rosie Mangan and Douglas Downie and Julie Coetzee and Martin Hill and Ashley Burke and Paul DOWNEY and Thomas Henry and Stephe Compton",
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    Paterson, I, Mangan, R, Downie, D, Coetzee, J, Hill, M, Burke, A, DOWNEY, P, Henry, T & Compton, S 2016, 'Two in one: cryptic species discovered in biological control agent populations using molecular data and crossbreeding experiments', Ecology and Evolution, vol. 6, no. 17, pp. 6139-6150. https://doi.org/10.1002/ece3.2297

    Two in one: cryptic species discovered in biological control agent populations using molecular data and crossbreeding experiments. / Paterson, Iain; Mangan, Rosie; Downie, Douglas; Coetzee, Julie; Hill, Martin; Burke, Ashley; DOWNEY, Paul; Henry, Thomas; Compton, Stephe.

    In: Ecology and Evolution, Vol. 6, No. 17, 2016, p. 6139-6150.

    Research output: Contribution to journalArticle

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    T1 - Two in one: cryptic species discovered in biological control agent populations using molecular data and crossbreeding experiments

    AU - Paterson, Iain

    AU - Mangan, Rosie

    AU - Downie, Douglas

    AU - Coetzee, Julie

    AU - Hill, Martin

    AU - Burke, Ashley

    AU - DOWNEY, Paul

    AU - Henry, Thomas

    AU - Compton, Stephe

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    AB - There are many examples of cryptic species that have been identified through DNA-barcoding or other genetic techniques. There are, however, very few confirmations of cryptic species being reproductively isolated. This study presents one of the few cases of cryptic species that has been confirmed to be reproductively isolated and therefore true species according to the biological species concept. The cryptic species are of special interest because they were discovered within biological control agent populations. Two geographically isolated populations of Eccritotarsus catarinensis (Carvalho) [Hemiptera: Miridae], a biological control agent for the invasive aquatic macrophyte, water hyacinth, Eichhornia crassipes (Mart.) Solms [Pontederiaceae], in South Africa, were sampled from the native range of the species in South America. Morphological characteristics indicated that both populations were the same species according to the current taxonomy, but subsequent DNA analysis and breeding experiments revealed that the two populations are reproductively isolated. Crossbreeding experiments resulted in very few hybrid offspring when individuals were forced to interbreed with individuals of the other population, and no hybrid offspring were recorded when a choice of mate from either population was offered. The data indicate that the two populations are cryptic species that are reproductively incompatible. Subtle but reliable diagnostic characteristics were then identified to distinguish between the two species which would have been considered intraspecific variation without the data from the genetics and interbreeding experiments. These findings suggest that all consignments of biological control agents from allopatric populations should be screened for cryptic species using genetic techniques and that the importation of multiple consignments of the same species for biological control should be conducted with caution.

    KW - Biological species concept

    KW - C01

    KW - DNA-barcoding

    KW - Eccritotarsus catarinensis

    KW - Eichhornia crassipes

    KW - Inter Simple Sequence Repeats

    KW - reproductive incompatibility

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