Type I and type II interferon responses in two human liver cell lines (Huh-7 and HuH6)

O Grunvogel, K Esser-Nobis, Marc Windisch, Michael FRESE, Martin Trippler, Ralf Bartenschlager, Volker Lohmann, Marco Binder

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Abstract

Most studies investigating the biology of Hepatitis C virus (HCV) have used the human hepatoma cell line Huh-7 or subclones thereof, as these are the most permissive cell lines for HCV infection and replication. Other cell lines also support replication of HCV, most notably the human hepatoblastoma cell line HuH6. HCV replication in cell culture is generally highly sensitive to interferons (IFNs) and differences in the IFN-mediated inhibition of virus replication may reflect alterations in the IFN-induced antiviral response inherent to different host cells. For example, HCV replication is highly sensitive to IFN-¿ treatment in Huh-7, but not in HuH6 cells. In this study, we used microarray-based gene expression profiling to compare the response of Huh-7 and HuH6 cells to stimulation with IFN-a and IFN-¿. Furthermore, we determined whether the resistance of HCV replication in HuH6 cells can be linked to differences in the expression profile of IFN-regulated genes. Although both cells lines responded to IFNs with rapid changes in gene expression, thereby demonstrating functional type I and type II signaling pathways, differences were observed for a number of genes. Raw and normalized expression data have been deposited in GEO under accession number GSE68927.
Original languageEnglish
Pages (from-to)166-170
Number of pages5
JournalGenomics Data
Volume7
DOIs
Publication statusPublished - 1 Mar 2016

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Interferon Type I
Liver
Interferons
Interferon-gamma
Viruses
Cells
Hepacivirus
Virus Replication
Cell Line
Gene expression
Genes
Hepatoblastoma
Gene Expression Profiling
Virus Diseases
Microarrays
Cell culture
Antiviral Agents
Hepatocellular Carcinoma
Cell Culture Techniques
Gene Expression

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Grunvogel, O., Esser-Nobis, K., Windisch, M., FRESE, M., Trippler, M., Bartenschlager, R., ... Binder, M. (2016). Type I and type II interferon responses in two human liver cell lines (Huh-7 and HuH6). Genomics Data, 7, 166-170. https://doi.org/10.1016/j.gdata.2015.12.017
Grunvogel, O ; Esser-Nobis, K ; Windisch, Marc ; FRESE, Michael ; Trippler, Martin ; Bartenschlager, Ralf ; Lohmann, Volker ; Binder, Marco. / Type I and type II interferon responses in two human liver cell lines (Huh-7 and HuH6). In: Genomics Data. 2016 ; Vol. 7. pp. 166-170.
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Grunvogel, O, Esser-Nobis, K, Windisch, M, FRESE, M, Trippler, M, Bartenschlager, R, Lohmann, V & Binder, M 2016, 'Type I and type II interferon responses in two human liver cell lines (Huh-7 and HuH6)', Genomics Data, vol. 7, pp. 166-170. https://doi.org/10.1016/j.gdata.2015.12.017

Type I and type II interferon responses in two human liver cell lines (Huh-7 and HuH6). / Grunvogel, O; Esser-Nobis, K; Windisch, Marc; FRESE, Michael; Trippler, Martin; Bartenschlager, Ralf; Lohmann, Volker; Binder, Marco.

In: Genomics Data, Vol. 7, 01.03.2016, p. 166-170.

Research output: Contribution to journalArticle

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Grunvogel O, Esser-Nobis K, Windisch M, FRESE M, Trippler M, Bartenschlager R et al. Type I and type II interferon responses in two human liver cell lines (Huh-7 and HuH6). Genomics Data. 2016 Mar 1;7:166-170. https://doi.org/10.1016/j.gdata.2015.12.017