Uptake and fate of ethephon (2-chloroethylphosphonic acid) in dormant weed seeds

J. STEPHEN GOUDEY, Deep Saini, M. S. SPENCER

Research output: Contribution to journalArticle

Abstract

Although ethephon ([2-chloroethyl]phosphonic acid) is often used as a form of liquid ethylene in studies of seed germination, it is not known if ethylene evolved from ethephon in the seed is sufficient to elicit the desired response and/or if ethephon has a regulatory action that alone accounts for the response. For these reasons we studied the uptake and fate of [1,2-(14)C]ethephon in dormant seeds of Avena fatua, Sinapis arvensis, Thlaspi arvense, and Chenopodium album. The radioactivity within the seeds was separated into a labile carbon-labeled ethephon/ethylene fraction (64-87%) and, following extraction in methanol-chloroform-water (12:5:3), into fractions associated with insoluble (12-29%) and soluble (3-8%) seed constituents. The radioactivity associated with seed constituents was reduced 5 to 75% by hot alkaline hydrolysis (2.5 n KOH, 70 degrees C for 1 hour). Although a small portion of the ethephon (or metabolite of ethephon/ethylene) taken up by the seeds is tightly bound to the tissues, our results indicate that, at the appropriate external concentrations of ethephon, the amount of ethylene evolved from ethephon within the seeds is sufficient to produce the desired ethylene mediated responses. However, factors affecting the decomposition of ethephon must be considered in the decision as to whether to use ethephon as a liquid supply of ethylene
LanguageEnglish
Pages155-157
Number of pages3
JournalPlant Physiology
Volume85
Issue number1
DOIs
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

ethephon
Seeds
weeds
uptake mechanisms
ethylene
seeds
Radioactivity
Thlaspi
Chenopodium album
Sinapis
Thlaspi arvense
Sinapis arvensis
phosphorous acid
Avena fatua
liquids
Chloroform
Germination
chloroform
Methanol
seed germination

Cite this

GOUDEY, J. STEPHEN ; Saini, Deep ; SPENCER, M. S. / Uptake and fate of ethephon (2-chloroethylphosphonic acid) in dormant weed seeds. In: Plant Physiology. 1987 ; Vol. 85, No. 1. pp. 155-157.
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title = "Uptake and fate of ethephon (2-chloroethylphosphonic acid) in dormant weed seeds",
abstract = "Although ethephon ([2-chloroethyl]phosphonic acid) is often used as a form of liquid ethylene in studies of seed germination, it is not known if ethylene evolved from ethephon in the seed is sufficient to elicit the desired response and/or if ethephon has a regulatory action that alone accounts for the response. For these reasons we studied the uptake and fate of [1,2-(14)C]ethephon in dormant seeds of Avena fatua, Sinapis arvensis, Thlaspi arvense, and Chenopodium album. The radioactivity within the seeds was separated into a labile carbon-labeled ethephon/ethylene fraction (64-87{\%}) and, following extraction in methanol-chloroform-water (12:5:3), into fractions associated with insoluble (12-29{\%}) and soluble (3-8{\%}) seed constituents. The radioactivity associated with seed constituents was reduced 5 to 75{\%} by hot alkaline hydrolysis (2.5 n KOH, 70 degrees C for 1 hour). Although a small portion of the ethephon (or metabolite of ethephon/ethylene) taken up by the seeds is tightly bound to the tissues, our results indicate that, at the appropriate external concentrations of ethephon, the amount of ethylene evolved from ethephon within the seeds is sufficient to produce the desired ethylene mediated responses. However, factors affecting the decomposition of ethephon must be considered in the decision as to whether to use ethephon as a liquid supply of ethylene",
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Uptake and fate of ethephon (2-chloroethylphosphonic acid) in dormant weed seeds. / GOUDEY, J. STEPHEN; Saini, Deep; SPENCER, M. S.

In: Plant Physiology, Vol. 85, No. 1, 1987, p. 155-157.

Research output: Contribution to journalArticle

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AU - GOUDEY, J. STEPHEN

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AU - SPENCER, M. S.

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AB - Although ethephon ([2-chloroethyl]phosphonic acid) is often used as a form of liquid ethylene in studies of seed germination, it is not known if ethylene evolved from ethephon in the seed is sufficient to elicit the desired response and/or if ethephon has a regulatory action that alone accounts for the response. For these reasons we studied the uptake and fate of [1,2-(14)C]ethephon in dormant seeds of Avena fatua, Sinapis arvensis, Thlaspi arvense, and Chenopodium album. The radioactivity within the seeds was separated into a labile carbon-labeled ethephon/ethylene fraction (64-87%) and, following extraction in methanol-chloroform-water (12:5:3), into fractions associated with insoluble (12-29%) and soluble (3-8%) seed constituents. The radioactivity associated with seed constituents was reduced 5 to 75% by hot alkaline hydrolysis (2.5 n KOH, 70 degrees C for 1 hour). Although a small portion of the ethephon (or metabolite of ethephon/ethylene) taken up by the seeds is tightly bound to the tissues, our results indicate that, at the appropriate external concentrations of ethephon, the amount of ethylene evolved from ethephon within the seeds is sufficient to produce the desired ethylene mediated responses. However, factors affecting the decomposition of ethephon must be considered in the decision as to whether to use ethephon as a liquid supply of ethylene

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