Urinary Hydroxyproline Is Only Suitable As a Biomarker for Acute Intake, Up to 6 hr Postingestion of Collagen Proteins in "Free-Living," Healthy, Active Males

Rebekah D. Alcock, Gregory C. Shaw, Nicolin Tee, Marijke Welvaert, Louise M. Burke

Research output: Contribution to journalArticle

Abstract

The urinary excretion of hydroxyproline (Hyp), abundant in collagen protein, may serve as a biomarker of habitual collagen intake, assisting with investigations of current interest in the role of dietary collagen intake in supporting the synthesis of collagenous body tissues. This study investigated the time course of urinary Hyp excretion in "free-living," healthy, active males following the ingestion of a standardized bolus (20 g) of collagenous (gelatin and a hydrolyzed collagen powder) and dairy (calcium caseinate and hydrolyzed casein) proteins. The excretion of Hyp was assessed over a 24-hr period, separated into three collection periods: 0-6, 6-12, and 12-24 hr. Hyp was elevated for 0-6 hr after the consumption of collagen-containing supplements (gelatin 31.3 ± 8.8 mmol/mol and hydrolyzed collagen 33.7 ± 22.0 mmol/mol vs. baseline: gelatin 2.4 ± 1.7 mmol/mol and hydrolyzed collagen 2.8 ± 1.5 mmol/mol; p < .05), but not for the dairy protein supplements (calcium caseinate 3.4 ± 1.7 mmol/mol and hydrolyzed casein 4.0 ± 3.7 mmol/mol; p > .05). Therefore, urinary Hyp reflects an acute intake of collagenous protein, but is not suitable as a biomarker for quantifying habitual collagen intake, provided through regular dietary practices in "free-living," healthy, active males.

Original languageEnglish
Pages (from-to)461-465
Number of pages5
JournalInternational Journal of Sport Nutrition and Exercise Metabolism
Volume29
Issue number5
DOIs
Publication statusPublished - 2019

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Hydroxyproline
Collagen
Biomarkers
Proteins
Gelatin
Caseins
Powders
Eating

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title = "Urinary Hydroxyproline Is Only Suitable As a Biomarker for Acute Intake, Up to 6 hr Postingestion of Collagen Proteins in {"}Free-Living,{"} Healthy, Active Males",
abstract = "The urinary excretion of hydroxyproline (Hyp), abundant in collagen protein, may serve as a biomarker of habitual collagen intake, assisting with investigations of current interest in the role of dietary collagen intake in supporting the synthesis of collagenous body tissues. This study investigated the time course of urinary Hyp excretion in {"}free-living,{"} healthy, active males following the ingestion of a standardized bolus (20 g) of collagenous (gelatin and a hydrolyzed collagen powder) and dairy (calcium caseinate and hydrolyzed casein) proteins. The excretion of Hyp was assessed over a 24-hr period, separated into three collection periods: 0-6, 6-12, and 12-24 hr. Hyp was elevated for 0-6 hr after the consumption of collagen-containing supplements (gelatin 31.3 ± 8.8 mmol/mol and hydrolyzed collagen 33.7 ± 22.0 mmol/mol vs. baseline: gelatin 2.4 ± 1.7 mmol/mol and hydrolyzed collagen 2.8 ± 1.5 mmol/mol; p < .05), but not for the dairy protein supplements (calcium caseinate 3.4 ± 1.7 mmol/mol and hydrolyzed casein 4.0 ± 3.7 mmol/mol; p > .05). Therefore, urinary Hyp reflects an acute intake of collagenous protein, but is not suitable as a biomarker for quantifying habitual collagen intake, provided through regular dietary practices in {"}free-living,{"} healthy, active males.",
keywords = "connective tissue, gelatin, ligament, nutrition, tendon, Sports Nutritional Physiological Phenomena, Eating, Collagen/administration & dosage, Humans, Male, Cross-Over Studies, Hydroxyproline/urine, Caseins, Biomarkers/urine, Adult, Dietary Supplements",
author = "Alcock, {Rebekah D.} and Shaw, {Gregory C.} and Nicolin Tee and Marijke Welvaert and Burke, {Louise M.}",
year = "2019",
doi = "10.1123/ijsnem.2019-0024",
language = "English",
volume = "29",
pages = "461--465",
journal = "International Journal of Sport Nutrition",
issn = "1526-484X",
publisher = "Human Kinetics Publishers Inc.",
number = "5",

}

Urinary Hydroxyproline Is Only Suitable As a Biomarker for Acute Intake, Up to 6 hr Postingestion of Collagen Proteins in "Free-Living," Healthy, Active Males. / Alcock, Rebekah D.; Shaw, Gregory C.; Tee, Nicolin; Welvaert, Marijke; Burke, Louise M.

In: International Journal of Sport Nutrition and Exercise Metabolism, Vol. 29, No. 5, 2019, p. 461-465.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Urinary Hydroxyproline Is Only Suitable As a Biomarker for Acute Intake, Up to 6 hr Postingestion of Collagen Proteins in "Free-Living," Healthy, Active Males

AU - Alcock, Rebekah D.

AU - Shaw, Gregory C.

AU - Tee, Nicolin

AU - Welvaert, Marijke

AU - Burke, Louise M.

PY - 2019

Y1 - 2019

N2 - The urinary excretion of hydroxyproline (Hyp), abundant in collagen protein, may serve as a biomarker of habitual collagen intake, assisting with investigations of current interest in the role of dietary collagen intake in supporting the synthesis of collagenous body tissues. This study investigated the time course of urinary Hyp excretion in "free-living," healthy, active males following the ingestion of a standardized bolus (20 g) of collagenous (gelatin and a hydrolyzed collagen powder) and dairy (calcium caseinate and hydrolyzed casein) proteins. The excretion of Hyp was assessed over a 24-hr period, separated into three collection periods: 0-6, 6-12, and 12-24 hr. Hyp was elevated for 0-6 hr after the consumption of collagen-containing supplements (gelatin 31.3 ± 8.8 mmol/mol and hydrolyzed collagen 33.7 ± 22.0 mmol/mol vs. baseline: gelatin 2.4 ± 1.7 mmol/mol and hydrolyzed collagen 2.8 ± 1.5 mmol/mol; p < .05), but not for the dairy protein supplements (calcium caseinate 3.4 ± 1.7 mmol/mol and hydrolyzed casein 4.0 ± 3.7 mmol/mol; p > .05). Therefore, urinary Hyp reflects an acute intake of collagenous protein, but is not suitable as a biomarker for quantifying habitual collagen intake, provided through regular dietary practices in "free-living," healthy, active males.

AB - The urinary excretion of hydroxyproline (Hyp), abundant in collagen protein, may serve as a biomarker of habitual collagen intake, assisting with investigations of current interest in the role of dietary collagen intake in supporting the synthesis of collagenous body tissues. This study investigated the time course of urinary Hyp excretion in "free-living," healthy, active males following the ingestion of a standardized bolus (20 g) of collagenous (gelatin and a hydrolyzed collagen powder) and dairy (calcium caseinate and hydrolyzed casein) proteins. The excretion of Hyp was assessed over a 24-hr period, separated into three collection periods: 0-6, 6-12, and 12-24 hr. Hyp was elevated for 0-6 hr after the consumption of collagen-containing supplements (gelatin 31.3 ± 8.8 mmol/mol and hydrolyzed collagen 33.7 ± 22.0 mmol/mol vs. baseline: gelatin 2.4 ± 1.7 mmol/mol and hydrolyzed collagen 2.8 ± 1.5 mmol/mol; p < .05), but not for the dairy protein supplements (calcium caseinate 3.4 ± 1.7 mmol/mol and hydrolyzed casein 4.0 ± 3.7 mmol/mol; p > .05). Therefore, urinary Hyp reflects an acute intake of collagenous protein, but is not suitable as a biomarker for quantifying habitual collagen intake, provided through regular dietary practices in "free-living," healthy, active males.

KW - connective tissue

KW - gelatin

KW - ligament

KW - nutrition

KW - tendon

KW - Sports Nutritional Physiological Phenomena

KW - Eating

KW - Collagen/administration & dosage

KW - Humans

KW - Male

KW - Cross-Over Studies

KW - Hydroxyproline/urine

KW - Caseins

KW - Biomarkers/urine

KW - Adult

KW - Dietary Supplements

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U2 - 10.1123/ijsnem.2019-0024

DO - 10.1123/ijsnem.2019-0024

M3 - Article

VL - 29

SP - 461

EP - 465

JO - International Journal of Sport Nutrition

JF - International Journal of Sport Nutrition

SN - 1526-484X

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ER -