Use of cellulose acetate electrophoresis and nondestructive sampling procedures for identification of potential gene markers

P.B. Mather, W.A. Ruscoe

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Five species of freshwater fish that are native to Australia and are currently used for aquaculture or are being considered for future development as cultured species – golden perch (Macquaria ambigua), silver perch (Bidyanus bidyanus), spangled perch (Leipotherapon unicolor), Murray cod (Maccullochella peeli), and dewfish (Tandanus tandanus) – were sampled for cellulose acetate gel electrophoresis. Baseline data on enzyme loci were produced to detect which ones have potential use as gene markers. Twenty-six enzymes were screened for each of the five species, and the specific electrophoretic running conditions resolved 40 enzyme loci. Three types of tissue that did not require destructive sampling were examined (fin tissue, white muscle, and body mucus) to assess whether the products of enzyme loci could be detected. Some of the enzyme loci described from liver, white muscle, and eye tissues were resolved from each of these three tissues that can be nondestructively sampled. Thus, these tissues could allow for electrophoretic development of gene markers for individual species without harm to important broodstock individuals.
    Original languageUndefined
    Pages (from-to)446-449
    Number of pages4
    JournalProgressive Fish-Culturist
    Volume54
    Issue number4
    DOIs
    Publication statusPublished - 1992

    Cite this

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    title = "Use of cellulose acetate electrophoresis and nondestructive sampling procedures for identification of potential gene markers",
    abstract = "Five species of freshwater fish that are native to Australia and are currently used for aquaculture or are being considered for future development as cultured species – golden perch (Macquaria ambigua), silver perch (Bidyanus bidyanus), spangled perch (Leipotherapon unicolor), Murray cod (Maccullochella peeli), and dewfish (Tandanus tandanus) – were sampled for cellulose acetate gel electrophoresis. Baseline data on enzyme loci were produced to detect which ones have potential use as gene markers. Twenty-six enzymes were screened for each of the five species, and the specific electrophoretic running conditions resolved 40 enzyme loci. Three types of tissue that did not require destructive sampling were examined (fin tissue, white muscle, and body mucus) to assess whether the products of enzyme loci could be detected. Some of the enzyme loci described from liver, white muscle, and eye tissues were resolved from each of these three tissues that can be nondestructively sampled. Thus, these tissues could allow for electrophoretic development of gene markers for individual species without harm to important broodstock individuals.",
    author = "P.B. Mather and W.A. Ruscoe",
    note = "cited By 5",
    year = "1992",
    doi = "10.1577/1548-8640(1992)054<0246:UOCAEA>2.3.CO;2",
    language = "Undefined",
    volume = "54",
    pages = "446--449",
    journal = "Progressive Fish-Culturist",
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    Use of cellulose acetate electrophoresis and nondestructive sampling procedures for identification of potential gene markers. / Mather, P.B.; Ruscoe, W.A.

    In: Progressive Fish-Culturist, Vol. 54, No. 4, 1992, p. 446-449.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Use of cellulose acetate electrophoresis and nondestructive sampling procedures for identification of potential gene markers

    AU - Mather, P.B.

    AU - Ruscoe, W.A.

    N1 - cited By 5

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    Y1 - 1992

    N2 - Five species of freshwater fish that are native to Australia and are currently used for aquaculture or are being considered for future development as cultured species – golden perch (Macquaria ambigua), silver perch (Bidyanus bidyanus), spangled perch (Leipotherapon unicolor), Murray cod (Maccullochella peeli), and dewfish (Tandanus tandanus) – were sampled for cellulose acetate gel electrophoresis. Baseline data on enzyme loci were produced to detect which ones have potential use as gene markers. Twenty-six enzymes were screened for each of the five species, and the specific electrophoretic running conditions resolved 40 enzyme loci. Three types of tissue that did not require destructive sampling were examined (fin tissue, white muscle, and body mucus) to assess whether the products of enzyme loci could be detected. Some of the enzyme loci described from liver, white muscle, and eye tissues were resolved from each of these three tissues that can be nondestructively sampled. Thus, these tissues could allow for electrophoretic development of gene markers for individual species without harm to important broodstock individuals.

    AB - Five species of freshwater fish that are native to Australia and are currently used for aquaculture or are being considered for future development as cultured species – golden perch (Macquaria ambigua), silver perch (Bidyanus bidyanus), spangled perch (Leipotherapon unicolor), Murray cod (Maccullochella peeli), and dewfish (Tandanus tandanus) – were sampled for cellulose acetate gel electrophoresis. Baseline data on enzyme loci were produced to detect which ones have potential use as gene markers. Twenty-six enzymes were screened for each of the five species, and the specific electrophoretic running conditions resolved 40 enzyme loci. Three types of tissue that did not require destructive sampling were examined (fin tissue, white muscle, and body mucus) to assess whether the products of enzyme loci could be detected. Some of the enzyme loci described from liver, white muscle, and eye tissues were resolved from each of these three tissues that can be nondestructively sampled. Thus, these tissues could allow for electrophoretic development of gene markers for individual species without harm to important broodstock individuals.

    U2 - 10.1577/1548-8640(1992)054<0246:UOCAEA>2.3.CO;2

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