Validation and quantitation of an in-vitro M-cell model

Peter Tyrer, Alice Foxwell, Jenelle Maree KYD, Phillip Sizer

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    This study has evaluated an in vitro model of the follicle-associated epithelia that overlie Peyer's patches of the small intestine. The model shares many phenotypic characteristics of M cells in vivo. Co-cultures of the human adenocarcinoma cell line Caco-2 and freshly isolated Peyer's patch cells were established. Fluorescence microscopy and quantitative image analysis were used to validate the model against known markers of M-cell phenotype. Apical expression of alkaline phosphatase was down-regulated in co-cultures and villin was re-distributed from the apical membrane to the cytoplasm. alpha5beta1 integrin was found on the apical surfaces of the monolayers and B and T lymphocytes integrated into the monolayers. Particle transport was temperature-dependent in co-cultures, indicating that a transcytotic route was responsible. This model provides opportunities to study factors that influence M-cell development, assess putative Peyer's patch targeting in oral vaccine technologies, and study intestinal uptake in vitro
    Original languageEnglish
    Pages (from-to)377-383
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume299
    Issue number3
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Peyer's Patches
    Coculture Techniques
    Monolayers
    Integrin alpha5beta1
    T-cells
    Fluorescence microscopy
    Fluorescence Microscopy
    Cell culture
    Image analysis
    Small Intestine
    Alkaline Phosphatase
    Cytoplasm
    Adenocarcinoma
    B-Lymphocytes
    Vaccines
    Epithelium
    Cells
    Technology
    Membranes
    T-Lymphocytes

    Cite this

    Tyrer, Peter ; Foxwell, Alice ; KYD, Jenelle Maree ; Sizer, Phillip. / Validation and quantitation of an in-vitro M-cell model. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 299, No. 3. pp. 377-383.
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    author = "Peter Tyrer and Alice Foxwell and KYD, {Jenelle Maree} and Phillip Sizer",
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    Validation and quantitation of an in-vitro M-cell model. / Tyrer, Peter; Foxwell, Alice; KYD, Jenelle Maree; Sizer, Phillip.

    In: Biochemical and Biophysical Research Communications, Vol. 299, No. 3, 2002, p. 377-383.

    Research output: Contribution to journalArticle

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    AB - This study has evaluated an in vitro model of the follicle-associated epithelia that overlie Peyer's patches of the small intestine. The model shares many phenotypic characteristics of M cells in vivo. Co-cultures of the human adenocarcinoma cell line Caco-2 and freshly isolated Peyer's patch cells were established. Fluorescence microscopy and quantitative image analysis were used to validate the model against known markers of M-cell phenotype. Apical expression of alkaline phosphatase was down-regulated in co-cultures and villin was re-distributed from the apical membrane to the cytoplasm. alpha5beta1 integrin was found on the apical surfaces of the monolayers and B and T lymphocytes integrated into the monolayers. Particle transport was temperature-dependent in co-cultures, indicating that a transcytotic route was responsible. This model provides opportunities to study factors that influence M-cell development, assess putative Peyer's patch targeting in oral vaccine technologies, and study intestinal uptake in vitro

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