Variation in the nuclear effects of infection by different human rhinovirus serotypes

Erin WALKER, Sarah Croft, Reena GHILDYAL

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Human rhinovirus (HRV) is a positive sense RNA virus, which, despite replicating in the cytoplasm, has a significant impact on nuclear transport and nuclear localization of host proteins. A number of studies have identified differences between HRV serotypes, with respect to host response, protease activity and replicative ability. Here we report the sero-specific effects of two group-A HRV serotypes, the minor group HRV2 and the major group HRV16, on nuclear transport and nuclear protein localization. Using Western analysis, immunofluorescence and real time PCR, we show that HRV2 replicates at a faster rate than HRV16, which correlates with earlier production of viral proteases and disruption of host nuclear transport. There is significant variation in the nuclear effects of different rhinovirus species, which in turn may impact disease progression and patient response.
Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalFrontiers in Microbiology
Volume6
Issue numberAUG
DOIs
Publication statusPublished - 24 Aug 2015

Fingerprint

Rhinovirus
Cell Nucleus Active Transport
Infection
Peptide Hydrolases
RNA Viruses
Nuclear Proteins
Fluorescent Antibody Technique
Disease Progression
Real-Time Polymerase Chain Reaction
Carrier Proteins
Cytoplasm
Serogroup
Proteins

Cite this

@article{bdf914ab12fe4ad98bdbd991bc84ae35,
title = "Variation in the nuclear effects of infection by different human rhinovirus serotypes",
abstract = "Human rhinovirus (HRV) is a positive sense RNA virus, which, despite replicating in the cytoplasm, has a significant impact on nuclear transport and nuclear localization of host proteins. A number of studies have identified differences between HRV serotypes, with respect to host response, protease activity and replicative ability. Here we report the sero-specific effects of two group-A HRV serotypes, the minor group HRV2 and the major group HRV16, on nuclear transport and nuclear protein localization. Using Western analysis, immunofluorescence and real time PCR, we show that HRV2 replicates at a faster rate than HRV16, which correlates with earlier production of viral proteases and disruption of host nuclear transport. There is significant variation in the nuclear effects of different rhinovirus species, which in turn may impact disease progression and patient response.",
keywords = "Human rhinovirus, Nuclear transport, Nucleoporin, Serotype, Viral proteases",
author = "Erin WALKER and Sarah Croft and Reena GHILDYAL",
year = "2015",
month = "8",
day = "24",
doi = "10.3389/fmicb.2015.00875",
language = "English",
volume = "6",
pages = "1--10",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",
number = "AUG",

}

Variation in the nuclear effects of infection by different human rhinovirus serotypes. / WALKER, Erin; Croft, Sarah; GHILDYAL, Reena.

In: Frontiers in Microbiology, Vol. 6, No. AUG, 24.08.2015, p. 1-10.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Variation in the nuclear effects of infection by different human rhinovirus serotypes

AU - WALKER, Erin

AU - Croft, Sarah

AU - GHILDYAL, Reena

PY - 2015/8/24

Y1 - 2015/8/24

N2 - Human rhinovirus (HRV) is a positive sense RNA virus, which, despite replicating in the cytoplasm, has a significant impact on nuclear transport and nuclear localization of host proteins. A number of studies have identified differences between HRV serotypes, with respect to host response, protease activity and replicative ability. Here we report the sero-specific effects of two group-A HRV serotypes, the minor group HRV2 and the major group HRV16, on nuclear transport and nuclear protein localization. Using Western analysis, immunofluorescence and real time PCR, we show that HRV2 replicates at a faster rate than HRV16, which correlates with earlier production of viral proteases and disruption of host nuclear transport. There is significant variation in the nuclear effects of different rhinovirus species, which in turn may impact disease progression and patient response.

AB - Human rhinovirus (HRV) is a positive sense RNA virus, which, despite replicating in the cytoplasm, has a significant impact on nuclear transport and nuclear localization of host proteins. A number of studies have identified differences between HRV serotypes, with respect to host response, protease activity and replicative ability. Here we report the sero-specific effects of two group-A HRV serotypes, the minor group HRV2 and the major group HRV16, on nuclear transport and nuclear protein localization. Using Western analysis, immunofluorescence and real time PCR, we show that HRV2 replicates at a faster rate than HRV16, which correlates with earlier production of viral proteases and disruption of host nuclear transport. There is significant variation in the nuclear effects of different rhinovirus species, which in turn may impact disease progression and patient response.

KW - Human rhinovirus

KW - Nuclear transport

KW - Nucleoporin

KW - Serotype

KW - Viral proteases

UR - http://purl.org/au-research/grants/nhmrc/1027312

UR - http://www.scopus.com/inward/record.url?scp=84940930324&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2015.00875

DO - 10.3389/fmicb.2015.00875

M3 - Article

VL - 6

SP - 1

EP - 10

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - AUG

ER -