Vkorc1 sequencing suggests anticoagulant resistance in rats in New Zealand

P Cowan, Dianne GLEESON, R. Howitt, Ana Ramón-Laca, Alexandra Esther, Hans Joachim Pelz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

BACKGROUND: Anticoagulant toxins are used globally to control rats. Resistance of Rattus species to these toxins now occurs in at least 18 countries in Europe, America and Asia. Resistance is often associated with single nucleotide polymorphisms (SNPs) in the Vkorc1 gene. This study gives a first overview of the distribution and frequency of Vkorc1 SNPs in rats in New Zealand. New Zealand is unusual in having no native rodents but three species of introduced Rattus – norvegicus Berk., rattus L. and exulans Peale. RESULTS: Sequence variants occurred in at least one species of rat at all 30 of the sites sampled. Three new SNPs were identified, one in kiore and two in ship rats. No SNPs previously associated with resistance were found in Norway rats or kiore, but seven ship rats were heterozygous and one homozygous for the A74T variant. Its resultant Tyr25Phe mutation has previously been associated with resistance to both first- and second-generation anticoagulants in ship rats in Spain. CONCLUSIONS: This is the first evidence of potential resistance to anticoagulant toxins in rats in New Zealand. Further testing using blood clotting response times in dosed rats is needed to confirm resistance potentially conferred by the Tyr25Phe mutation. Assessment is also needed of the potential of the other non-synonymous variants (Ala14Val, Ala26Val) recorded in this study to confer resistance to anticoagulant toxins.
Original languageEnglish
Pages (from-to)262-266
Number of pages5
JournalPest Management Science
Volume73
Issue number1
DOIs
Publication statusPublished - Jan 2017

Fingerprint

anticoagulants
New Zealand
Anticoagulants
rats
single nucleotide polymorphism
toxins
ships
Rattus
Single Nucleotide Polymorphism
Ships
Rattus norvegicus
mutation
blood coagulation
Introduced Species
Mutation
rodents
Spain
Blood Coagulation
Reaction Time
Rodentia

Cite this

Cowan, P., GLEESON, D., Howitt, R., Ramón-Laca, A., Esther, A., & Pelz, H. J. (2017). Vkorc1 sequencing suggests anticoagulant resistance in rats in New Zealand. Pest Management Science, 73(1), 262-266. https://doi.org/10.1002/ps.4304
Cowan, P ; GLEESON, Dianne ; Howitt, R. ; Ramón-Laca, Ana ; Esther, Alexandra ; Pelz, Hans Joachim. / Vkorc1 sequencing suggests anticoagulant resistance in rats in New Zealand. In: Pest Management Science. 2017 ; Vol. 73, No. 1. pp. 262-266.
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abstract = "BACKGROUND: Anticoagulant toxins are used globally to control rats. Resistance of Rattus species to these toxins now occurs in at least 18 countries in Europe, America and Asia. Resistance is often associated with single nucleotide polymorphisms (SNPs) in the Vkorc1 gene. This study gives a first overview of the distribution and frequency of Vkorc1 SNPs in rats in New Zealand. New Zealand is unusual in having no native rodents but three species of introduced Rattus – norvegicus Berk., rattus L. and exulans Peale. RESULTS: Sequence variants occurred in at least one species of rat at all 30 of the sites sampled. Three new SNPs were identified, one in kiore and two in ship rats. No SNPs previously associated with resistance were found in Norway rats or kiore, but seven ship rats were heterozygous and one homozygous for the A74T variant. Its resultant Tyr25Phe mutation has previously been associated with resistance to both first- and second-generation anticoagulants in ship rats in Spain. CONCLUSIONS: This is the first evidence of potential resistance to anticoagulant toxins in rats in New Zealand. Further testing using blood clotting response times in dosed rats is needed to confirm resistance potentially conferred by the Tyr25Phe mutation. Assessment is also needed of the potential of the other non-synonymous variants (Ala14Val, Ala26Val) recorded in this study to confer resistance to anticoagulant toxins.",
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Cowan, P, GLEESON, D, Howitt, R, Ramón-Laca, A, Esther, A & Pelz, HJ 2017, 'Vkorc1 sequencing suggests anticoagulant resistance in rats in New Zealand', Pest Management Science, vol. 73, no. 1, pp. 262-266. https://doi.org/10.1002/ps.4304

Vkorc1 sequencing suggests anticoagulant resistance in rats in New Zealand. / Cowan, P; GLEESON, Dianne; Howitt, R.; Ramón-Laca, Ana; Esther, Alexandra; Pelz, Hans Joachim.

In: Pest Management Science, Vol. 73, No. 1, 01.2017, p. 262-266.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Vkorc1 sequencing suggests anticoagulant resistance in rats in New Zealand

AU - Cowan, P

AU - GLEESON, Dianne

AU - Howitt, R.

AU - Ramón-Laca, Ana

AU - Esther, Alexandra

AU - Pelz, Hans Joachim

PY - 2017/1

Y1 - 2017/1

N2 - BACKGROUND: Anticoagulant toxins are used globally to control rats. Resistance of Rattus species to these toxins now occurs in at least 18 countries in Europe, America and Asia. Resistance is often associated with single nucleotide polymorphisms (SNPs) in the Vkorc1 gene. This study gives a first overview of the distribution and frequency of Vkorc1 SNPs in rats in New Zealand. New Zealand is unusual in having no native rodents but three species of introduced Rattus – norvegicus Berk., rattus L. and exulans Peale. RESULTS: Sequence variants occurred in at least one species of rat at all 30 of the sites sampled. Three new SNPs were identified, one in kiore and two in ship rats. No SNPs previously associated with resistance were found in Norway rats or kiore, but seven ship rats were heterozygous and one homozygous for the A74T variant. Its resultant Tyr25Phe mutation has previously been associated with resistance to both first- and second-generation anticoagulants in ship rats in Spain. CONCLUSIONS: This is the first evidence of potential resistance to anticoagulant toxins in rats in New Zealand. Further testing using blood clotting response times in dosed rats is needed to confirm resistance potentially conferred by the Tyr25Phe mutation. Assessment is also needed of the potential of the other non-synonymous variants (Ala14Val, Ala26Val) recorded in this study to confer resistance to anticoagulant toxins.

AB - BACKGROUND: Anticoagulant toxins are used globally to control rats. Resistance of Rattus species to these toxins now occurs in at least 18 countries in Europe, America and Asia. Resistance is often associated with single nucleotide polymorphisms (SNPs) in the Vkorc1 gene. This study gives a first overview of the distribution and frequency of Vkorc1 SNPs in rats in New Zealand. New Zealand is unusual in having no native rodents but three species of introduced Rattus – norvegicus Berk., rattus L. and exulans Peale. RESULTS: Sequence variants occurred in at least one species of rat at all 30 of the sites sampled. Three new SNPs were identified, one in kiore and two in ship rats. No SNPs previously associated with resistance were found in Norway rats or kiore, but seven ship rats were heterozygous and one homozygous for the A74T variant. Its resultant Tyr25Phe mutation has previously been associated with resistance to both first- and second-generation anticoagulants in ship rats in Spain. CONCLUSIONS: This is the first evidence of potential resistance to anticoagulant toxins in rats in New Zealand. Further testing using blood clotting response times in dosed rats is needed to confirm resistance potentially conferred by the Tyr25Phe mutation. Assessment is also needed of the potential of the other non-synonymous variants (Ala14Val, Ala26Val) recorded in this study to confer resistance to anticoagulant toxins.

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KW - R. norvegicus

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KW - anticoagulant

KW - genetics

KW - resistance

KW - R.norvegicus

KW - R.exulans

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UR - http://www.mendeley.com/research/vkorc1-sequencing-suggests-anticoagulant-resistance-rats-new-zealand

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DO - 10.1002/ps.4304

M3 - Article

VL - 73

SP - 262

EP - 266

JO - Pesticide Science

JF - Pesticide Science

SN - 1526-498X

IS - 1

ER -