Water-stress-induced inhibition of α-tubulin gene expression during growth, and its implications for reproductive success in rice

Inder S. Sheoran, Priyum Koonjul, Jihad Attieh, Hargurdeep S. Saini

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A drought-suppressed cDNA (RiP-3), encoding a putative α-tubulin protein was isolated from rice panicle at pollen-mother-cell meiosis stage. Analysis of its deduced amino acid sequence showed all the typical structural motifs for plant α-tubulins. The expression of α-tubulin transcripts was observed in all the reproductive organs of rice panicle, and in 5- or 15-day old seedlings, but not in mature leaves. Expression levels were positively correlated with the regions and periods of high growth, and the transcript level declined in parallel with drought-induced reduction in growth rates in all tissues examined. Immunoblot analysis of proteins separated by SDS-PAGE with anti-α-tubulin monoclonal antibody showed that the level of protein paralleled the changes in the transcript abundance in these organs. In situ immunolocalization of the α-tubulin protein in sections of the basal part of 5-day old seedlings showed that the highest levels of the protein were associated with the fastest growing leaf whorls, and the protein level declined upon a brief episode of water stress. Given the known critical role of tubulin in cell division and elongation, the results indicate that the expression of α-tubulin gene may be part of the events that suppress panicle elongation during water deficit, which is in turn a suspected cause of male reproductive failure and yield reduction in rice.

Original languageEnglish
Pages (from-to)291-299
Number of pages9
JournalPlant Physiology and Biochemistry
Volume80
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

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Tubulin
tubulin
Dehydration
water stress
Gene Expression
rice
gene expression
Growth
Proteins
proteins
Droughts
Seedlings
drought
seedlings
Meiosis
Pollen
Oryza
reproductive success
meiosis
Cell Division

Cite this

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title = "Water-stress-induced inhibition of α-tubulin gene expression during growth, and its implications for reproductive success in rice",
abstract = "A drought-suppressed cDNA (RiP-3), encoding a putative α-tubulin protein was isolated from rice panicle at pollen-mother-cell meiosis stage. Analysis of its deduced amino acid sequence showed all the typical structural motifs for plant α-tubulins. The expression of α-tubulin transcripts was observed in all the reproductive organs of rice panicle, and in 5- or 15-day old seedlings, but not in mature leaves. Expression levels were positively correlated with the regions and periods of high growth, and the transcript level declined in parallel with drought-induced reduction in growth rates in all tissues examined. Immunoblot analysis of proteins separated by SDS-PAGE with anti-α-tubulin monoclonal antibody showed that the level of protein paralleled the changes in the transcript abundance in these organs. In situ immunolocalization of the α-tubulin protein in sections of the basal part of 5-day old seedlings showed that the highest levels of the protein were associated with the fastest growing leaf whorls, and the protein level declined upon a brief episode of water stress. Given the known critical role of tubulin in cell division and elongation, the results indicate that the expression of α-tubulin gene may be part of the events that suppress panicle elongation during water deficit, which is in turn a suspected cause of male reproductive failure and yield reduction in rice.",
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Water-stress-induced inhibition of α-tubulin gene expression during growth, and its implications for reproductive success in rice. / Sheoran, Inder S.; Koonjul, Priyum; Attieh, Jihad; Saini, Hargurdeep S.

In: Plant Physiology and Biochemistry, Vol. 80, 01.01.2014, p. 291-299.

Research output: Contribution to journalArticle

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