The number of threatened species continues to increase due to a range of anthropogenic disturbances, and many species continue to decline increasing their risk of extinction. Translocation is a widely used management technique to establish new populations to reduce the risk of extinction. There are, however, a range of issues to be considered. For example, for some species the donor population may be impacted by translocation, for other species it must be decided whether to translocate adults or juveniles to establish new populations. The question then becomes who do you move? The endangered Macquarie perch in south-eastern Australia is continuing to decline, with the recent Millennium Drought (1997-2010) and associated events (e.g., bushfires) contributing to dramatic local declines and the need for emergency responses. Successful historic translocations of this species involved adult fish, however the removal of significant numbers of adult fish may now impact source populations and alternative translocation approaches needed investigating. The use of sub-adult or juvenile fish, that would be expected to experience higher mortality, may be an approach to establishing new populations which would have less severe impacts on source populations. However, the number of fish required, frequency of translocation and likelihood of population establishment are unknown. This study outlines the development of a population model to assist in trialling translocation scenarios for establishing new populations of Macquarie perch. The model predicts that translocations of young-of-year fish (age 0+) is unlikely to be successful unless ~600 females are released annually for five years. If translocating yearling (age 1+) fish, annual translocations of >100 females is required to achieve success, with stocking for at least five consecutive years required. If the frequency of recruitment failure or magnitude of Allee effects increases, then translocations of increased numbers of yearlings or prolonged stocking (10 years) is required to achieve success. The addition of small numbers of adult fish in combination with yearlings decreases the number of yearlings required, and increases the chance of success under more stressful scenarios.