AbstractMicrosatellites are simple repetitive DNA sequences that are used as genetic markers throughout the biological sciences. The high levels of variation observed at microsatellite loci contribute to their utility in studies at the population and individual levels. This variation is a consequence of mutations that change the length of microsatellite repeat tracts. Current understanding suggests that most mutations are caused by polymerase slippage during DNA replication and lead to changes of a single repeat unit in length,but some changes involving multiple repeats can also occur. Despite this simplistic overview,there is evidence for considerable heterogeneity in mutation processes between species,loci and alleles. Such complex patterns suggest that other mechanisms,including those associated with DNA recombination,are also involved in the generation of microsatellite mutations. Understanding which mutational mechanisms are responsible for variation at microsatellite markers is essential to enable accurate data interpretation in genotyping projects,as many commonly used statistics assume specific mutation models. I developed microsatellite markers specific to the X and Y chromosomes and an autosome in the tammar wallaby,Macropus eugenii,and investigated their evolutionary properties using two approaches: indirectly,as inferred from population data,and directly,from observation of mutation events. First,I found that allelic richness increased with repeat length and that two popular mutation models,the stepwise mutation model and the infinite allele model,were poor at predicting the number of alleles per locus,particularly when gene diversity was high. These results suggest that neither model can account for all mutations at tammar wallaby microsatellites and hint at the involvement of more complex mechanisms than replication slippage. I also determined levels of variation at each locus in two tammar wallaby populations. I found that allelic richness was highest for chromosome 2,intermediate for the X chromosome and lowest for the Y chromosome in both populations. Thus,allelic richness varied between chromosomes in the manner predicted by their relative exposure to recombination,although these results may also be explained by the relative effective population sizes of the chromosomes studied. Second,I used small-pool PCR from sperm DNA to observe de novo mutation events at three of the most polymorphic autosomal markers. To determine the reliability of my observations I developed and applied strict criteria for scoring alleles and mutations at microsatellite loci. I observed mutations at all three markers,with rate variation between loci. Single step mutations could not be distinguished because of the limitations of the approach,but 24 multi-step mutations,involving changes of up to 35 repeat units,were recorded. Many of these mutations involved changes that could not be explained by the gain or loss of whole repeat units. These results imply that a large number of mutations at tammar wallaby microsatellites are caused by mechanisms other than replication slippage and are consistent with a role for recombination in the mutation process. Taken as a whole,my results provide evidence for complex mutation processes at tammar wallaby microsatellites. I conclude that careful characterisation of microsatellite mutation properties should be conducted on a case-by-case basis to determine the most appropriate mutation models and analysis tools for each locus. In addition,my work has provided a set of chromosome-specific markers for use in macropod genetic studies,which includes the first marsupial Y chromosome microsatellites. Sex chromosome microsatellites open a new range of possibilities for population studies,as they provide opportunities to investigate gene flow in a male context,to complement data from autosomal and maternally-inherited mitochondrial markers.
|Date of Award||1 Jan 2008|
Sex chromosome microsatellite markers from an Australian marsupial: development, application and evolution
MacDonald, A. J. (Author). 1 Jan 2008
Student thesis: Doctoral Thesis